Sandbox Reserved 427
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| This Sandbox is Reserved from January 19, 2016, through August 31, 2016 for use for Proteopedia Team Projects by the class Chemistry 423 Biochemistry for Chemists taught by Lynmarie K Thompson at University of Massachusetts Amherst, USA. This reservation includes Sandbox Reserved 425 through Sandbox Reserved 439. |
Contents |
Vitamin D binding protein (1j7e)
Student Projects for UMass Chemistry 423 Spring 2016
Introduction
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This is a sample scene created with SAT to by Group, and another to make of the protein.
Vitamin D binding protein Overview:
1) purposes
2) History (various name changes since discovery)
3) overview of structure:
green scenes for , ball and stick, secondary structure
4) Brief mention of unique binding characteristics.
5) Therapeutic uses
Overall Structure
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This scene highlights the in pink in the protein and the binding sights in green.
Binding Interactions
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Additional Features
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Synthesis
Synthesized in the liver. Will also include details about folding, posttranslational modification and chaperone proteins, if any.
Actin Binding Interactions
Vitamin D binding protein is also capable of interacting with actin at the domains shown in . This function occurs mainly in the bloodstream, as DBP binds to globular actin present in the plasma. It presents an important mechanism for clearing actin from necrotic or apoptotic tissue (Meier et all 2006)
Other Interactions
"Macrophage modulation Chemotaxis of C5 derived peptides Transport of fatty acids and endotoxins Inhibition of platelet induced aggregation Osteoclast Activation" from Meier et al 2006 Figure 2
Role in Disease
Altered levels in hepatic failure, AHF, trauma, immune function. Deficient mice show no phenotype. White and Cooke.
Other Notable Ligands
Quiz Question 1
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Vitamin D binding protein is very similar to based on sequence similarity as well as an almost identical tertiary structure. However, HSA binds to actin instead, and is actually unable to bind to Vitamin D3. Based on what you have learned about the binding nature in domain I of Vitamin D Binding Protein, hypothesize a reason why HSA is unable to bind to Vitamin D3, but Vitamin D binding protein can. The following scenes should help guide your thinking = Domain I Helices 2,3,4 HSA; Domain I Helices 2,3,4 Vitamin D binding Protein.
See Also
Credits
Introduction - Uday Prakhya
Overall Structure - Elizabeth Swanson
Drug Binding Site - Alex Debreceni
Additional Features - Nick Rivelli
Quiz Question 1 - Robert Green
References
[1] Gomme PT, Bertolini J. 2004. Therapeutic potential of vitamin D-binding protein. Trends Biotechnol. 22:340–345.
[2] Haddad JG. 1995. Plasma vitamin D-binding protein (Gc-globulin): Multiple tasks. J. Steroid Biochem. Mol. Biol. 53:579–582.
[3] Otterbein LR, Cosio C, Graceffa P, Dominguez R. 2002. Crystal structures of the vitamin D-binding protein and its complex with actin: structural basis of the actin-scavenger system. Proc. Natl. Acad. Sci. U. S. A. 99:8003–8008.
[4] Speeckaert M, Huang G, Delanghe JR, Taes YEC. 2006. Biological and clinical aspects of the vitamin D binding protein (Gc-globulin) and its polymorphism. Clin. Chim. Acta 372:33–42.
[5] Verboven C, Rabijns A, De Maeyer M, Van Baelen H, Bouillon R, De Ranter C. 2002. A structural basis for the unique binding features of the human vitamin D-binding protein. Nat. Struct. Biol. 9:131–6.
[6] White P, Cooke N. 2000. The multifunctional properties and characteristics of vitamin D-binding protein. Trends Endocrinol. Metab. 11:320–327.
