Sandbox Reserved 431

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This Sandbox is Reserved from January 19, 2016, through August 31, 2016 for use for Proteopedia Team Projects by the class Chemistry 423 Biochemistry for Chemists taught by Lynmarie K Thompson at University of Massachusetts Amherst, USA. This reservation includes Sandbox Reserved 425 through Sandbox Reserved 439.

Contents 1 Vitamin D activation by cytochrome P450, rickets (3c6g)[1] 2 Introduction 3 Overall Structure 4 Binding Interactions 5 Additional Features 6 Quiz Question 1 7 See Also 8 Credits 9 References

Vitamin D activation by cytochrome P450, rickets (3c6g)^[1]

by Isabel Hand, Elizabeth Humble, Kati Johnson, Samantha Kriksceonaitis, and Matthew Tiller

Student Projects for UMass Chemistry 423 Spring 2016

Introduction

spinqualitylabelsall models pdbcode, Insert caption here Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Overall Structure

spinqualitylabelsall models 3c6g, insert caption here Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

- Asymmetric dimer
- Consists of α-helices, β-sheets (mostly on one side of the molecule) with a heme buried inside the protein
- Two molecules of 2-hydroxypropyl-β-cyclodextrin are found near the dimer interface

Binding Interactions

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-catalyzes initial step for converting vitamin D into 25-hydroxyvitamin D -mutation causes rickets-25-hydroxylase deficiency -has closed conformation, substrate access channel mostly covered -secosteroid binding, extended active site

Additional Features

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This molecule has a heme which is bound to iron, which, combined with its structural conformation, allows for hydroxylation with the attached substrate. This molecule carries out important functions and is not species or sex specific.

Quiz Question 1

(merely an example of what this section might look like)

spinqualitylabelsall models pdbcode, Insert caption here Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

from can you identify the green, red, and blue parts of the molecule?

See Also

• Cytochrome P450
• Drug Metabolism by CYP450 Enzymes
• 3w0y
• 3w0c

Credits

Introduction - Sami Kriksceonaitis

Overall Structure - Kati Johnson

Drug Binding Site - Isabel Hand

Additional Features - Elizabeth Humble

Quiz Question 1 - Matthew Tiller

References

1. ↑ Strushkevich N, Usanov SA, Plotnikov AN, Jones G, Park HW. Structural analysis of CYP2R1 in complex with vitamin D3. J Mol Biol. 2008 Jun 27;380(1):95-106. Epub 2008 Apr 8. PMID:18511070 doi:10.1016/j.jmb.2008.03.065