Sandbox Wabash 21 Fumarase

The enzyme known as fumarase catalyzes the conversion of malate to fumarase. Crystallographic studies using inhibitors revealed that the inhibitors bound to two different locations. This indicated that there were two potential active sites for fumarase. Site A was located within a pit and was made up of atoms from 3 of the 4 subunits present within fumarase. Site B was located towards the surface of the enzyme and was made up of only 1 of the 4 subunits. Debate about which of the sites was the true active site was centered around the fact that there was no known monomeric fumarase. Since site A was made up of atoms from multiple subunits, site A seemed like the likely active site for fumarase. This was tested by mutating the catalytic His on both of the sites and observing the amount of fumarase activity.

Function

Disease

Relevance

Structural highlights

This is a sample scene created with SAT to by Group, and another to make of the protein. You can make your own scenes on SAT starting from scratch or loading and editing one of these sample scenes.