Fumarase C is the stable fumarase found in E. coli acting as a "non-iron-containing enzyme" found in eukaryotic cells. Fumarase is a vital enzyme in eukaryotic cells because it has the ability to catalyze dehydration and hydration reactions between L-malate and fumarate,[1] a reaction found in the Krebs Cycle.
Active Site Debate
| Header Sample
| Header Site Linked to
| Header Average Activity (μ/mL)
| Header Specific Activity (μ/mg)
|
| Wild Type
| NA
| 4920
| 116
|
| H129N
| B Site
| 2080
| 143
|
| H188N
| A Site
| 10
| 0.6
|
Structure of Active Site
References
- ↑ Weaver T, Lees M, Banaszak L. Mutations of fumarase that distinguish between the active site and a nearby dicarboxylic acid binding site. Protein Sci. 1997 Apr;6(4):834-42. PMID:9098893
