Galactose oxidase

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Template:STRUCTURE 1gog

Contents 1 Function 2 Structural highlights 3 3D structures of galactose oxidase 4 References

Function

Galactose oxidase (GAO) catalyzes the oxidation of D-galactose using O2 to D-galacto-hexodialdose and hydrogen peroxide. GAO catalytic motif is a free radical-coupled copper^[1].

Structural highlights

GAO uses copper and active site post-translational cross-linking of Cys228 and Tyr272 via a thioester bond for activity^[2].

3D structures of galactose oxidase

Updated on 03-March-2016

1gof, 1gog, 1goh – GAO – Hypomyces rosellus
1k3i – FuGAO precursor – Fusarium
1t2x – FuGAO (mutant)
2eib, 2eic, 2eid, 2wq8 - GzGAO (mutant) – Gibberella zeae
2eie – GzGAO + N3
2jkx – GzGAO no Cu
2vz1 – GzGAO premature
2vz3 - GzGAO

References

1. ↑ Baron AJ, Stevens C, Wilmot C, Seneviratne KD, Blakeley V, Dooley DM, Phillips SE, Knowles PF, McPherson MJ. Structure and mechanism of galactose oxidase. The free radical site. J Biol Chem. 1994 Oct 7;269(40):25095-105. PMID:7929198
2. ↑ Ito N, Phillips SE, Stevens C, Ogel ZB, McPherson MJ, Keen JN, Yadav KD, Knowles PF. Novel thioether bond revealed by a 1.7 A crystal structure of galactose oxidase. Nature. 1991 Mar 7;350(6313):87-90. PMID:2002850 doi:http://dx.doi.org/10.1038/350087a0