Glutamate synthase

From Proteopedia

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spinqualitylabelsall models Caption for this structure Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image Contents 1 Function 2 Disease 3 Relevance 4 Structural highlights Function Glutamate synthase (GS) catalyzes the reverse reaction which converts L-glutamine, 2-oxo-glutarate and NADPH to L-glutarate and NADP. [1]. Fd-dependent glutamate synthase catalyzes the reverse reaction converting L-glutamate and oxidized ferredoxin to L-glutamine, 2-oxo-glutarate and oxidized ferredoxin[2]. Disease Relevance Structural highlights

• Glutamate synthase

• • 1ea0 – AbGS alpha subunit + FMN + oxo-glutarate – Azospirillum brasilense
    
  • 2vdc – AbGS alpha+beta subunits + FAD + FMN + oxo-glutarate – CryoEM
    

• Fd-dependent glutamate synthase

• • 1llw, 1ofd – SyGS + FMN + oxo-glutarate – Synechocystis
    
  • 1llz, 1lm1 – SyGS + FMN
    
  • 1ofe – SyGS + FMN + oxglutarate + oxo-norleucine
    

References

1. ↑ Vanoni MA, Curti B. Structure-function studies of glutamate synthases: a class of self-regulated iron-sulfur flavoenzymes essential for nitrogen assimilation. IUBMB Life. 2008 May;60(5):287-300. doi: 10.1002/iub.52. PMID:18421771 doi:http://dx.doi.org/10.1002/iub.52
2. ↑ Hirasawa M, Tamura G. Flavin and iron-sulfur containing ferredoxin-linked glutamate synthase from spinach leaves. J Biochem. 1984 Apr;95(4):983-94. PMID:6746604