5l3r

Structural highlights

Function

[SR54C_ARATH] Involved in cotranslational and post-translational sorting of thylakoid proteins. Binds GTP specifically. Activates the GTPase activity of CPFTSY when bound together. Required for light-harvesting chlorophyll a/b-binding protein (LHCP) integration into thylakoids.^{[1] [2] [3] [4]} [CFTSY_ARATH] Signal recognition particle receptor protein. Binds GTP specifically. The GTPase activity is inhibited by the N-terminus of the protein until binding to the thylakoid membrane. Activates the GTPase activity of FFC/cpSRP54 when bound to the cpSRP complex. Required for light-harvesting chlorophyll a/b-binding protein (LHCP) integration into thylakoids. Might be also functionally linked to the Sec translocation machinery.^{[5] [6] [7] [8] [9] [10]}

References

1. ↑ Tu CJ, Schuenemann D, Hoffman NE. Chloroplast FtsY, chloroplast signal recognition particle, and GTP are required to reconstitute the soluble phase of light-harvesting chlorophyll protein transport into thylakoid membranes. J Biol Chem. 1999 Sep 17;274(38):27219-24. PMID:10480939
2. ↑ Groves MR, Mant A, Kuhn A, Koch J, Dubel S, Robinson C, Sinning I. Functional characterization of recombinant chloroplast signal recognition particle. J Biol Chem. 2001 Jul 27;276(30):27778-86. Epub 2001 May 16. PMID:11356852 doi:http://dx.doi.org/10.1074/jbc.M103470200
3. ↑ Jaru-Ampornpan P, Chandrasekar S, Shan SO. Efficient interaction between two GTPases allows the chloroplast SRP pathway to bypass the requirement for an SRP RNA. Mol Biol Cell. 2007 Jul;18(7):2636-45. Epub 2007 May 2. PMID:17475780 doi:http://dx.doi.org/10.1091/mbc.E07-01-0037
4. ↑ Rutschow H, Ytterberg AJ, Friso G, Nilsson R, van Wijk KJ. Quantitative proteomics of a chloroplast SRP54 sorting mutant and its genetic interactions with CLPC1 in Arabidopsis. Plant Physiol. 2008 Sep;148(1):156-75. doi: 10.1104/pp.108.124545. Epub 2008 Jul , 16. PMID:18633119 doi:http://dx.doi.org/10.1104/pp.108.124545
5. ↑ Kogata N, Nishio K, Hirohashi T, Kikuchi S, Nakai M. Involvement of a chloroplast homologue of the signal recognition particle receptor protein, FtsY, in protein targeting to thylakoids. FEBS Lett. 1999 Mar 26;447(2-3):329-33. PMID:10214972
6. ↑ Tu CJ, Schuenemann D, Hoffman NE. Chloroplast FtsY, chloroplast signal recognition particle, and GTP are required to reconstitute the soluble phase of light-harvesting chlorophyll protein transport into thylakoid membranes. J Biol Chem. 1999 Sep 17;274(38):27219-24. PMID:10480939
7. ↑ Yuan J, Kight A, Goforth RL, Moore M, Peterson EC, Sakon J, Henry R. ATP stimulates signal recognition particle (SRP)/FtsY-supported protein integration in chloroplasts. J Biol Chem. 2002 Aug 30;277(35):32400-4. Epub 2002 Jun 24. PMID:12105232 doi:http://dx.doi.org/10.1074/jbc.M206192200
8. ↑ Jaru-Ampornpan P, Chandrasekar S, Shan SO. Efficient interaction between two GTPases allows the chloroplast SRP pathway to bypass the requirement for an SRP RNA. Mol Biol Cell. 2007 Jul;18(7):2636-45. Epub 2007 May 2. PMID:17475780 doi:http://dx.doi.org/10.1091/mbc.E07-01-0037
9. ↑ Asakura Y, Kikuchi S, Nakai M. Non-identical contributions of two membrane-bound cpSRP components, cpFtsY and Alb3, to thylakoid biogenesis. Plant J. 2008 Dec;56(6):1007-17. doi: 10.1111/j.1365-313X.2008.03659.x. Epub 2008, Aug 21. PMID:18764927 doi:http://dx.doi.org/10.1111/j.1365-313X.2008.03659.x
10. ↑ Marty NJ, Rajalingam D, Kight AD, Lewis NE, Fologea D, Kumar TK, Henry RL, Goforth RL. The membrane-binding motif of the chloroplast signal recognition particle receptor (cpFtsY) regulates GTPase activity. J Biol Chem. 2009 May 29;284(22):14891-903. doi: 10.1074/jbc.M900775200. Epub, 2009 Mar 17. PMID:19293157 doi:http://dx.doi.org/10.1074/jbc.M900775200