Phosphotriesterase

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Contents 1 Function 2 Relevance 3 Structural highlights 4 3D structures of phosphotriesterase 5 References

Function

Phosphotriesterase or aryldialkylphosphatase (PTE) catalyzes the conversion of aryl dialkyl phosphate to dialkyl phosphate and aryl alcohol^[1].

Relevance

PTE has no known natural substrate but has potential in detoxification of organophosphates poisons lethal effects. PTE can detoxify the insecticide paraoxon and the chemical warfare agent sarin^[2].

Structural highlights

The reaction product of PTE is located in the binuclear metal center^[3].

3D structures of phosphotriesterase

Updated on 03-July-2016

References

1. ↑ Roodveldt C, Tawfik DS. Directed evolution of phosphotriesterase from Pseudomonas diminuta for heterologous expression in Escherichia coli results in stabilization of the metal-free state. Protein Eng Des Sel. 2005 Jan;18(1):51-8. PMID:15790580 doi:http://dx.doi.org/10.1093/protein/gzi005
2. ↑ Bigley AN, Mabanglo MF, Harvey SP, Raushel FM. Variants of Phosphotriesterase for the Enhanced Detoxification of the Chemical Warfare Agent VR. Biochemistry. 2015 Aug 25. PMID:26274608 doi:http://dx.doi.org/10.1021/acs.biochem.5b00629
3. ↑ Kim J, Tsai PC, Chen SL, Himo F, Almo SC, Raushel FM. Structure of diethyl phosphate bound to the binuclear metal center of phosphotriesterase. Biochemistry. 2008 Sep 9;47(36):9497-504. Epub 2008 Aug 15. PMID:18702530 doi:10.1021/bi800971v