1jy6
From Proteopedia
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| Ligands: | |||||||
| Related: | 1JY4
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
B4DIMERA: A DE NOVO DESIGNED FOUR-STRANDED BETA-SHEET ASSEMBLED USING A DISULFIDE BOND
Overview
The design and characterization of an open eight-stranded beta-sheet in a synthetic, 2-fold symmetric 70-residue peptide is described. The design strategy involves the generation of a 35-residue four-stranded beta-sheet peptide in which successive hairpins are nucleated by appropriately positioned (D)Pro-Xxx sequences. Oxidative dimerization using a single Cys residue positioned at the center of the C-terminal strand results in a disulfide-bridged eight-stranded structure. Nuclear Overhauser effects firmly establish an eight-stranded beta-sheet in methanol. In water, the outer strands are frayed, but a well-defined four-stranded beta-sheet stabilized by a disulfide bridge and a hydrophobic cluster is determined from NMR data. Comparison of the precursor peptide with the disulfide-bridged dimer reveals considerable enhancement of beta-sheet content in the latter, suggesting that the disulfide cross-link is an effective strategy for the stabilization of beta-sheets.
About this Structure
1JY6 is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Design and construction of an open multistranded beta-sheet polypeptide stabilized by a disulfide bridge., Venkatraman J, Nagana Gowda GA, Balaram P, J Am Chem Soc. 2002 May 8;124(18):4987-94. PMID:11982362
Page seeded by OCA on Sun Mar 30 21:40:38 2008
