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Publication Abstract from PubMed

The truncated carbohydrate-active enzyme belonging to family 43 glycoside hydrolase from Clostridium thermocellum (CtGH43) is an alpha-L-arabinofuranosidase that in combination with endoxylanase leads to complete breakdown of L-arabinosyl-substituted xylans. The recombinant enzyme CtGH43 from C. thermocellum was overexpressed in Escherichia coli and purified by immobilized metal-ion affinity chromatography. The recombinant CtGH43 has a molecular mass of 35.86 kDa. Preliminary structural characterization was carried out on CtGH43 crystallized from different conditions, which gave either cube-shaped or brick-shaped crystals. These diffracted to a resolution of 1.65 A for the cubic form and 1.1 A for the monoclinic form. Molecular replacement was used to solve the CtGH43 structure.

Crystallization and preliminary X-ray crystallographic analysis of a novel alpha-L-arabinofuranosidase (CtGH43) from Clostridium thermocellum ATCC 27405.,Goyal A, Ahmed S, Fontes CM, Najmudin S Acta Crystallogr F Struct Biol Commun. 2014 May;70(Pt 5):616-8. doi:, 10.1107/S2053230X14006402. Epub 2014 Apr 15. PMID:24817722^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.