| Structural highlights
3axy is a 12 chain structure with sequence from Japanese rice. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
| | NonStd Res: | |
| Gene: | HD3A, LOC_Os06g06320, Os06g0157700, OsJ_20191, P0046E09.30, P0702F05.10 (Japanese rice), GF14C, LOC_Os08g33370, OJ1124_B05.7, Os08g0430500 (Japanese rice) |
| Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Function
[HD3A_ORYSJ] Probable mobile flower-promoting signal (florigen) that moves from the leaf to the shoot apical meristem (SAM) and induces flowering. Promotes the transition from vegetative growth to flowering downstream of HD1 and EHD1 under short day (SD) conditions. Acts upstream of MADS14 and MADS15.[1] [2] [3] [4] [14333_ORYSJ] Is associated with a DNA binding complex that binds to the G box, a well-characterized cis-acting DNA regulatory element found in plant genes.
Publication Abstract from PubMed
'Florigen' was proposed 75 years ago to be synthesized in the leaf and transported to the shoot apex, where it induces flowering. Only recently have genetic and biochemical studies established that florigen is encoded by FLOWERING LOCUS T (FT), a gene that is universally conserved in higher plants. Nonetheless, the exact function of florigen during floral induction remains poorly understood and receptors for florigen have not been identified. Here we show that the rice FT homologue Hd3a interacts with 14-3-3 proteins in the apical cells of shoots, yielding a complex that translocates to the nucleus and binds to the Oryza sativa (Os)FD1 transcription factor, a rice homologue of Arabidopsis thaliana FD. The resultant ternary 'florigen activation complex' (FAC) induces transcription of OsMADS15, a homologue of A. thaliana APETALA1 (AP1), which leads to flowering. We have determined the 2.4 A crystal structure of rice FAC, which provides a mechanistic basis for florigen function in flowering. Our results indicate that 14-3-3 proteins act as intracellular receptors for florigen in shoot apical cells, and offer new approaches to manipulate flowering in various crops and trees.
14-3-3 proteins act as intracellular receptors for rice Hd3a florigen.,Taoka K, Ohki I, Tsuji H, Furuita K, Hayashi K, Yanase T, Yamaguchi M, Nakashima C, Purwestri YA, Tamaki S, Ogaki Y, Shimada C, Nakagawa A, Kojima C, Shimamoto K Nature. 2011 Jul 31;476(7360):332-5. doi: 10.1038/nature10272. PMID:21804566[5]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Kojima S, Takahashi Y, Kobayashi Y, Monna L, Sasaki T, Araki T, Yano M. Hd3a, a rice ortholog of the Arabidopsis FT gene, promotes transition to flowering downstream of Hd1 under short-day conditions. Plant Cell Physiol. 2002 Oct;43(10):1096-105. PMID:12407188
- ↑ Tamaki S, Matsuo S, Wong HL, Yokoi S, Shimamoto K. Hd3a protein is a mobile flowering signal in rice. Science. 2007 May 18;316(5827):1033-6. Epub 2007 Apr 19. PMID:17446351 doi:http://dx.doi.org/10.1126/science.1141753
- ↑ Komiya R, Ikegami A, Tamaki S, Yokoi S, Shimamoto K. Hd3a and RFT1 are essential for flowering in rice. Development. 2008 Feb;135(4):767-74. doi: 10.1242/dev.008631. PMID:18223202 doi:http://dx.doi.org/10.1242/dev.008631
- ↑ Komiya R, Yokoi S, Shimamoto K. A gene network for long-day flowering activates RFT1 encoding a mobile flowering signal in rice. Development. 2009 Oct;136(20):3443-50. doi: 10.1242/dev.040170. Epub 2009 Sep 17. PMID:19762423 doi:http://dx.doi.org/10.1242/dev.040170
- ↑ Taoka K, Ohki I, Tsuji H, Furuita K, Hayashi K, Yanase T, Yamaguchi M, Nakashima C, Purwestri YA, Tamaki S, Ogaki Y, Shimada C, Nakagawa A, Kojima C, Shimamoto K. 14-3-3 proteins act as intracellular receptors for rice Hd3a florigen. Nature. 2011 Jul 31;476(7360):332-5. doi: 10.1038/nature10272. PMID:21804566 doi:10.1038/nature10272
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