1l0z
From Proteopedia
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| , resolution 1.5Å | |||||||
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| Ligands: | , , | ||||||
| Activity: | Pancreatic elastase, with EC number 3.4.21.36 | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
THE STRUCTURE OF PORCINE PANCREATIC ELASTASE COMPLEXED WITH XENON AND BROMIDE, CRYOPROTECTED WITH DRY PARAFFIN OIL
Overview
Crystals of porcine pancreatic elastase can be derived by soaking in high-molarity bromide solutions. These crystals, either in a glycerol-based cryoprotectant solution or in paraffin oil, can be subsequently pressurized under a xenon atmosphere to incorporate xenon. When paraffin oil is used, a larger number of bromide ions are observed on the protein surface. Intensity data collected to lower energy than the bromine absorption edge can be used to determine the xenon position and the resultant phase information can be used to determine the bromine substructure from data collected to higher energy than the bromine absorption edge. The method would appear to have general applicability where large substructures need to be determined.
About this Structure
1L0Z is a Single protein structure of sequence from Sus scrofa. Full crystallographic information is available from OCA.
Reference
Xenon derivatization of halide-soaked protein crystals., Panjikar S, Tucker PA, Acta Crystallogr D Biol Crystallogr. 2002 Sep;58(Pt 9):1413-20. Epub 2002, Aug 23. PMID:12198297
Page seeded by OCA on Sun Mar 30 21:56:29 2008
Categories: Pancreatic elastase | Single protein | Sus scrofa | Panjikar, S. | Tucker, P A. | Bromide | Ppe | Xenon
