Structural highlights
Publication Abstract from PubMed
Enterovirus 71 (EV71) is a picornavirus that causes outbreaks of hand foot and mouth disease (HFMD) primarily in the Asia-Pacific area. Unlike coxsackievirus A16, which also causes HFMD, EV71 induces severe neuropathology leading to high fatalities especially among children under the age of 6 years. Currently, no established vaccines or treatments are available against EV71 infection. The monoclonal antibody (MAb), MA28-7, neutralizes only specific strains of EV71 that have a conserved glycine at amino acid, VP1-145, a surface exposed residue that maps to the five-fold vertex, and has been implicated in receptor binding. The cryo-electron microscopy structure of a complex between EV71 and the Fab fragment of MA28-7 shows that only one Fab occupies each five-fold vertex. A positively-charged patch, which has also been implicated in receptor binding lies within the Fab footprint. We identify the strain-specific epitope of EV71 and discuss the possible neutralization mechanisms of the antibody.
A strain-specific epitope of Enterovirus 71 identified by cryoEM of the complex with Fab from neutralizing antibody.,Lee H, Cifuente JO, Ashley RE, Conway JF, Makhov AM, Tano Y, Shimizu H, Nishimura Y, Hafenstein S J Virol. 2013 Aug 14. PMID:23946455[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Lee H, Cifuente JO, Ashley RE, Conway JF, Makhov AM, Tano Y, Shimizu H, Nishimura Y, Hafenstein S. A strain-specific epitope of Enterovirus 71 identified by cryoEM of the complex with Fab from neutralizing antibody. J Virol. 2013 Aug 14. PMID:23946455 doi:10.1128/JVI.01926-13
