Structural highlights
3nzm is a 1 chain structure with sequence from Aphanocapsa sp. (strain n-1). Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
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| Ligands: | |
| NonStd Res: | |
| Related: | 1zde, 1zd7 |
| Gene: | dnaE-N, slr0603, dnaE-C, sll1572 (Aphanocapsa sp. (strain N-1)) |
| Activity: | DNA-directed DNA polymerase, with EC number 2.7.7.7 |
| Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Publication Abstract from PubMed
Here we describe self-splicing proteins, called inteins, that function as redox-responsive switches in bacteria. Redox regulation was achieved by engineering a disulfide bond between the intein's catalytic cysteine and a cysteine in the flanking 'extein' sequence. This interaction was validated by an X-ray structure, which includes a transient splice junction. A natural analog of the designed system was identified in Pyrococcus abyssi, suggesting an unprecedented form of adaptive, post-translational regulation.
Structure of catalytically competent intein caught in a redox trap with functional and evolutionary implications.,Callahan BP, Topilina NI, Stanger MJ, Van Roey P, Belfort M Nat Struct Mol Biol. 2011 May;18(5):630-3. Epub 2011 Apr 3. PMID:21460844[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Callahan BP, Topilina NI, Stanger MJ, Van Roey P, Belfort M. Structure of catalytically competent intein caught in a redox trap with functional and evolutionary implications. Nat Struct Mol Biol. 2011 May;18(5):630-3. Epub 2011 Apr 3. PMID:21460844 doi:10.1038/nsmb.2041
