Structural highlights
3qe4 is a 2 chain structure with sequence from Atcc 43067. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
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| Ligands: | |
| Related: | 2ag6, 1zh0, 1zh6 |
| Gene: | MJ0389, TyrRS, tyrS (ATCC 43067) |
| Activity: | Tyrosine--tRNA ligase, with EC number 6.1.1.1 |
| Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Function
[SYY_METJA] Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr).[1]
Publication Abstract from PubMed
We have employed a rapid fluorescence-based screen to assess the polyspecificity of several aminoacyl-tRNA synthetases (aaRSs) against an array of unnatural amino acids. We discovered that a p-cyanophenylalanine specific aminoacyl-tRNA synthetase (pCNF-RS) has high substrate permissivity for unnatural amino acids, while maintaining its ability to discriminate against the 20 canonical amino acids. This orthogonal pCNF-RS, together with its cognate amber nonsense suppressor tRNA, is able to selectively incorporate 18 unnatural amino acids into proteins, including trifluoroketone-, alkynyl-, and halogen-substituted amino acids. In an attempt to improve our understanding of this polyspecificity, the X-ray crystal structure of the aaRS-p-cyanophenylalanine complex was determined. A comparison of this structure with those of other mutant aaRSs showed that both binding site size and other more subtle features control substrate polyspecificity.
An Evolved Aminoacyl-tRNA Synthetase with Atypical Polysubstrate Specificity .,Young DD, Young TS, Jahnz M, Ahmad I, Spraggon G, Schultz PG Biochemistry. 2011 Feb 1. PMID:21280675[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Steer BA, Schimmel P. Major anticodon-binding region missing from an archaebacterial tRNA synthetase. J Biol Chem. 1999 Dec 10;274(50):35601-6. PMID:10585437
- ↑ Young DD, Young TS, Jahnz M, Ahmad I, Spraggon G, Schultz PG. An Evolved Aminoacyl-tRNA Synthetase with Atypical Polysubstrate Specificity . Biochemistry. 2011 Feb 1. PMID:21280675 doi:10.1021/bi101929e
