Structural highlights
Function
[IML3_YEAST] Component of the central kinetochore, which mediates the attachment of the centromere to the mitotic spindle by forming essential interactions between the microtubule-associated outer kinetochore proteins and the centromere-associated inner kinetochore proteins. Required for establishing bipolar spindle-microtubule attachments and proper chromosome segregation.[1] [2]
Publication Abstract from PubMed
Accurate segregation of genetic material in eukaryotes relies on the kinetochore, a multiprotein complex that connects centromeric DNA with microtubules. In yeast and humans, two proteins-Mif2/CENP-C and Chl4/CNEP-N-interact with specialized centromeric nucleosomes and establish distinct but cross-connecting axes of chromatin-microtubule linkage. Proteins recruited by Chl4/CENP-N include a subset that regulates chromosome transmission fidelity. We show that Chl4 and a conserved member of this subset, Iml3, both from Saccharomyces cerevisiae, form a stable protein complex that interacts with Mif2 and Sgo1. We have determined the structures of an Iml3 homodimer and an Iml3-Chl4 heterodimer, which suggest a mechanism for regulating the assembly of this functional axis of the kinetochore. We propose that at the core centromere, the Chl4-Iml3 complex participates in recruiting factors, such as Sgo1, that influence sister chromatid cohesion and encourage sister kinetochore biorientation.
An Iml3-Chl4 Heterodimer Links the Core Centromere to Factors Required for Accurate Chromosome Segregation.,Hinshaw SM, Harrison SC Cell Rep. 2013 Sep 25. pii: S2211-1247(13)00477-4. doi:, 10.1016/j.celrep.2013.08.036. PMID:24075991[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Ghosh SK, Poddar A, Hajra S, Sanyal K, Sinha P. The IML3/MCM19 gene of Saccharomyces cerevisiae is required for a kinetochore-related process during chromosome segregation. Mol Genet Genomics. 2001 Apr;265(2):249-57. PMID:11361335
- ↑ Ghosh SK, Sau S, Lahiri S, Lohia A, Sinha P. The Iml3 protein of the budding yeast is required for the prevention of precocious sister chromatid separation in meiosis I and for sister chromatid disjunction in meiosis II. Curr Genet. 2004 Aug;46(2):82-91. Epub 2004 Jul 6. PMID:15241623 doi:http://dx.doi.org/10.1007/s00294-004-0516-6
- ↑ Hinshaw SM, Harrison SC. An Iml3-Chl4 Heterodimer Links the Core Centromere to Factors Required for Accurate Chromosome Segregation. Cell Rep. 2013 Sep 25. pii: S2211-1247(13)00477-4. doi:, 10.1016/j.celrep.2013.08.036. PMID:24075991 doi:http://dx.doi.org/10.1016/j.celrep.2013.08.036
