Structural highlights
4eb5 is a 4 chain structure with sequence from Arcfu. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
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| Ligands: | , , , |
| Gene: | iscS2, AF_0564 (ARCFU), AF_0185, AF_0565 (ARCFU) |
| Activity: | Cysteine desulfurase, with EC number 2.8.1.7 |
| Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Function
[ISCS2_ARCFU] Catalyzes the removal of elemental sulfur from cysteine to produce alanine (By similarity). [ISCU1_ARCFU] A scaffold on which IscS assembles Fe-S clusters. Subsequently gives the nascent cluster to other proteins. It is likely that Fe-S cluster coordination is flexible as the role of this complex is to build and then hand off Fe-S clusters (By similarity).
Publication Abstract from PubMed
The center of attention: IscS cysteine desulfurases and IscU scaffolds are involved in biological iron-sulfur cluster assembly. The X-ray structure of an anaerobically produced, mutated (Fe(2) S(2) -(IscS-IscU(D35A) ))(2) complex reveals a cluster coordinated by three IscU cysteines and the IscS active cysteine. In air-exposed crystals the cluster is oxidized to an Fe(2) S-S center; D35 is essential for complex dissociation.
(IscS-IscU)(2) Complex Structures Provide Insights into Fe(2) S(2) Biogenesis and Transfer.,Marinoni EN, de Oliveira JS, Nicolet Y, Raulfs EC, Amara P, Dean DR, Fontecilla-Camps JC Angew Chem Int Ed Engl. 2012 Apr 18. doi: 10.1002/anie.201201708. PMID:22511353[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Marinoni EN, de Oliveira JS, Nicolet Y, Raulfs EC, Amara P, Dean DR, Fontecilla-Camps JC. (IscS-IscU)(2) Complex Structures Provide Insights into Fe(2) S(2) Biogenesis and Transfer. Angew Chem Int Ed Engl. 2012 Apr 18. doi: 10.1002/anie.201201708. PMID:22511353 doi:10.1002/anie.201201708
