Structural highlights
Function
[GPSM2_MOUSE] Plays an important role in spindle pole orientation (By similarity). Interacts and contributes to the functional activity of G(i) alpha proteins. Acts to stabilize the apical complex during neuroblast divisions. [FRPD1_HUMAN] Stabilizes membrane-bound GPSM1, and thereby promotes its interaction with GNAI1.[1]
Publication Abstract from PubMed
The tetratricopeptide repeat (TPR) motif-containing protein LGN binds multiple targets and regulates their subcellular localizations and functions during both asymmetric and symmetric cell divisions. Here, we characterized the interaction between LGN-TPR motifs and FERM and PDZ domain containing 1 (Frmpd1) and reported the crystal structure of the complex at 2.4A resolution. A highly conserved fragment at the center of Frmpd1 of ~20 residues was found to be necessary and sufficient to bind to LGN-TPR. This Frmpd1 fragment forms an extended structure and runs along the concave channel of the TPR superhelix in an antiparallel manner in the complex. Structural comparisons and biochemical studies of LGN/Frmpd1 and other known LGN/target interactions demonstrate that the LGN-TPR motifs are versatile and capable of recognizing multiple targets via diverse binding modes. Nevertheless, a conserved "E/QxEx4-5E/D/Qx1-2K/R" motif in LGN/Pins (partner of inscuteable) TPR binding proteins has been identified.
Structural and biochemical characterization of the interaction between LGN and Frmpd1.,Pan Z, Shang Y, Jia M, Zhang L, Xia C, Zhang M, Wang W, Wen W J Mol Biol. 2013 Mar 25;425(6):1039-49. doi: 10.1016/j.jmb.2013.01.003. Epub 2013, Jan 11. PMID:23318951[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ An N, Blumer JB, Bernard ML, Lanier SM. The PDZ and band 4.1 containing protein Frmpd1 regulates the subcellular location of activator of G-protein signaling 3 and its interaction with G-proteins. J Biol Chem. 2008 Sep 5;283(36):24718-28. Epub 2008 Jun 19. PMID:18566450 doi:M803497200
- ↑ Pan Z, Shang Y, Jia M, Zhang L, Xia C, Zhang M, Wang W, Wen W. Structural and biochemical characterization of the interaction between LGN and Frmpd1. J Mol Biol. 2013 Mar 25;425(6):1039-49. doi: 10.1016/j.jmb.2013.01.003. Epub 2013, Jan 11. PMID:23318951 doi:10.1016/j.jmb.2013.01.003
