1n6q
From Proteopedia
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| , resolution 3.Å | |||||||
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| Ligands: | , , , , , , | ||||||
| Gene: | POL (Human immunodeficiency virus 1) | ||||||
| Activity: | RNA-directed DNA polymerase, with EC number 2.7.7.49 | ||||||
| Related: | 1N5Y, 1RTD
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
HIV-1 Reverse Transcriptase Crosslinked to pre-translocation AZTMP-terminated DNA (complex N)
Overview
AZT (3'-azido-3'-deoxythymidine) resistance involves the enhanced excision of AZTMP from the end of the primer strand by HIV-1 reverse transcriptase. This reaction can occur when an AZTMP-terminated primer is bound at the nucleotide-binding site (pre-translocation complex N) but not at the 'priming' site (post-translocation complex P). We determined the crystal structures of N and P complexes at 3.0 and 3.1 A resolution. These structures provide insight into the structural basis of AZTMP excision and the mechanism of translocation. Docking of a dNTP in the P complex structure suggests steric crowding in forming a stable ternary complex that should increase the relative amount of the N complex, which is the substrate for excision. Structural differences between complexes N and P suggest that the conserved YMDD loop is involved in translocation, acting as a springboard that helps to propel the primer terminus from the N to the P site after dNMP incorporation.
About this Structure
1N6Q is a Protein complex structure of sequences from Human immunodeficiency virus 1 and Mus musculus. Full crystallographic information is available from OCA.
Reference
Structures of HIV-1 reverse transcriptase with pre- and post-translocation AZTMP-terminated DNA., Sarafianos SG, Clark AD Jr, Das K, Tuske S, Birktoft JJ, Ilankumaran P, Ramesha AR, Sayer JM, Jerina DM, Boyer PL, Hughes SH, Arnold E, EMBO J. 2002 Dec 2;21(23):6614-24. PMID:12456667
Page seeded by OCA on Sun Mar 30 22:26:10 2008
Categories: Human immunodeficiency virus 1 | Mus musculus | Protein complex | RNA-directed DNA polymerase | Arnold, E. | Birktoft, J J. | Boyer, P L. | Das, K. | Hughes, S H. | Ilankumaran, I. | Jerina, D M. | Jr., A D.Clark. | Ramesha, A R. | Sarafianos, S G. | Sayer, J M. | Tuske, S. | Complex n | Drug resistance | Hiv | Nucleotide excision | Pre-translocation | Reverse transcriptase | Translocation
