Structural highlights
4pk0 is a 2 chain structure with sequence from [1] and Actinoplanes teichomyceticus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
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| Ligands: | , , , , , , , |
| NonStd Res: | , , , , , , , , |
| Related: | 4pjz |
| Activity: | Lysozyme, with EC number 3.2.1.17 |
| Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Publication Abstract from PubMed
We report the X-ray crystal structure of a site-selective peptide catalyst moiety and teicoplanin A2-2 complex. The expressed protein ligation technique was used to couple T4 lysozyme (T4L) and a synthetic peptide catalyst responsible for the selective phosphorylation of the N-acetylglucosamine sugar in a teicoplanin A2-2 derivative. The T4L-Pmh-dPro-Aib-dAla-dAla construct was crystallized in the presence of teicoplanin A2-2. The resulting 2.3 A resolution protein-peptide-teicoplanin complex crystal structure revealed that the nucleophilic nitrogen of N-methylimidazole in the Pmh residue is in closer proximity (7.6 A) to the N-acetylglucosamine than the two other sugar rings present in teicoplanin (9.3 and 20.3 A, respectively). This molecular arrangement is consistent with the observed selectivity afforded by the peptide-based catalyst when it is applied to a site-selective phosphorylation reaction involving a teicoplanin A2-2 derivative.
X-ray Crystal Structure of Teicoplanin A-2 Bound to a Catalytic Peptide Sequence via the Carrier Protein Strategy.,Han S, Le BV, Hajare HS, Baxter RH, Miller SJ J Org Chem. 2014 Sep 2. PMID:25147913[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Han S, Le BV, Hajare HS, Baxter RH, Miller SJ. X-ray Crystal Structure of Teicoplanin A-2 Bound to a Catalytic Peptide Sequence via the Carrier Protein Strategy. J Org Chem. 2014 Sep 2. PMID:25147913 doi:http://dx.doi.org/10.1021/jo501625f
