1or7
From Proteopedia
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| , resolution 2.00Å | |||||||
|---|---|---|---|---|---|---|---|
| Gene: | RPOE OR SIGE OR B2573 OR C3097 OR Z3855 OR ECS3439 OR SF2635 (Escherichia coli), RSEA OR MCLA OR B2572 OR C3096 (Escherichia coli) | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of Escherichia coli sigmaE with the Cytoplasmic Domain of its Anti-sigma RseA
Overview
The sigma factors are the key regulators of bacterial transcription. ECF (extracytoplasmic function) sigma's are the largest and most divergent group of sigma(70) family members. ECF sigma's are normally sequestered in an inactive complex by their specific anti-sigma factor, which often spans the inner membrane. Here, we determined the 2 A resolution crystal structure of the Escherichia coli ECF sigma factor sigma(E) in an inhibitory complex with the cytoplasmic domain of its anti-sigma, RseA. Despite extensive sequence variability, the two major domains of sigma(E) are virtually identical in structure to the corresponding domains of other sigma(70) family members. In combination with a model of the sigma(E) holoenzyme and biochemical data, the structure reveals that RseA functions by sterically occluding the two primary binding determinants on sigma(E) for core RNA polymerase.
About this Structure
1OR7 is a Protein complex structure of sequences from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Crystal structure of Escherichia coli sigmaE with the cytoplasmic domain of its anti-sigma RseA., Campbell EA, Tupy JL, Gruber TM, Wang S, Sharp MM, Gross CA, Darst SA, Mol Cell. 2003 Apr;11(4):1067-78. PMID:12718891
Page seeded by OCA on Sun Mar 30 22:49:11 2008
