Function
Peptidyl-tRNA hydrolase (PTH) is a bacterial enzyme which cleaves peptidyl-tRNA to free peptide and tRNA[1]. PTH catalyzes the reaction in cases of aborted translation for recycling the tRNA for further rounds of protein synthesis. PTH is essential for bacterial viability.
Structural highlights
PTH dimer interacts with tRNA at the latter's CCA - the 3' end of the molecule which binds the amino acid; the acceptor site which pass-pairs tRNA N-terminal nucleotides to the C-terminal nucleotides and the TΨC site (Ψ is pseudouridine) which is the tRNA ribosome recognition site[2].
