1osl
From Proteopedia
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| Ligands: | , , , | ||||||
| Gene: | LACI (Escherichia coli) | ||||||
| Related: | 1L1M
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Solution structure of a dimeric lactose DNA-binding domain complexed to a nonspecific DNA sequence
Overview
Interaction of regulatory DNA binding proteins with their target sites is usually preceded by binding to nonspecific DNA. This speeds up the search for the target site by several orders of magnitude. We report the solution structure and dynamics of the complex of a dimeric lac repressor DNA binding domain with nonspecific DNA. The same set of residues can switch roles from a purely electrostatic interaction with the DNA backbone in the nonspecific complex to a highly specific binding mode with the base pairs of the cognate operator sequence. The protein-DNA interface of the nonspecific complex is flexible on biologically relevant time scales that may assist in the rapid and efficient finding of the target site.
About this Structure
1OSL is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Structure and flexibility adaptation in nonspecific and specific protein-DNA complexes., Kalodimos CG, Biris N, Bonvin AM, Levandoski MM, Guennuegues M, Boelens R, Kaptein R, Science. 2004 Jul 16;305(5682):386-9. PMID:15256668
Page seeded by OCA on Sun Mar 30 22:49:45 2008
