1pev
From Proteopedia
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| , resolution 2.00Å | |||||||
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| Gene: | UNC-78 OR C04F6.4 (Caenorhabditis elegans) | ||||||
| Domains: | WD40, COG2319 | ||||||
| Related: | 1NR0
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of the Actin Interacting Protein from Caenorhabditis Elegans
Overview
Actin-interacting protein 1 (AIP1) is a WD40 repeat protein that enhances actin filament disassembly in the presence of actin-depolymerizing factor (ADF)/cofilin. AIP1 also caps the barbed end of ADF/cofilin-bound actin filament. However, the mechanism by which AIP1 interacts with ADF/cofilin and actin is not clearly understood. We determined the crystal structure of Caenorhabditis elegans AIP1 (UNC-78), which revealed 14 WD40 modules arranged in two seven-bladed beta-propeller domains. The structure allowed for the mapping of conserved surface residues, and mutagenesis studies identified five residues that affected the ADF/cofilin-dependent actin filament disassembly activity. Mutations of these residues, which reside in blades 3 and 4 in the N-terminal propeller domain, had significant effects on the disassembly activity but did not alter the barbed end capping activity. These data support a model in which this conserved surface of AIP1 plays a direct role in enhancing fragmentation/depolymerization of ADF/cofilin-bound actin filaments but not in barbed end capping.
About this Structure
1PEV is a Single protein structure of sequence from Caenorhabditis elegans. Full crystallographic information is available from OCA.
Reference
Identification of functional residues on Caenorhabditis elegans actin-interacting protein 1 (UNC-78) for disassembly of actin depolymerizing factor/cofilin-bound actin filaments., Mohri K, Vorobiev S, Fedorov AA, Almo SC, Ono S, J Biol Chem. 2004 Jul 23;279(30):31697-707. Epub 2004 May 18. PMID:15150269
Page seeded by OCA on Sun Mar 30 22:58:42 2008
Categories: Caenorhabditis elegans | Single protein | Almo, S C. | Burley, S K. | Fedorov, A A. | Mohri, K. | NYSGXRC, New York Structural GenomiX Research Consortium. | Ono, S. | Vorobiev, S. | Actin interacting protein | Adf | Beta propeller | Cofilin | New york structural genomix research consortium | Nysgxrc | Protein structure initiative | Psi | Structural genomic | Wd40 repeat
