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1pp5

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Revision as of 20:02, 30 March 2008 by OCA (Talk | contribs)
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Image:1pp5.gif


PDB ID 1pp5

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Gene: MCJA (Escherichia coli)
Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



Structure of Antibacterial Peptide Microcin J25: a 21-Residue Lariat Protoknot


Overview

The antibacterial peptide microcin J25 (MccJ25) inhibits bacterial transcription by binding within, and obstructing, the nucleotide-uptake channel of bacterial RNA polymerase. Published covalent and three-dimensional structures indicate that MccJ25 is a 21-residue cycle. Here, we show that the published covalent and three-dimensional structures are incorrect, and that MccJ25 in fact is a 21-residue "lariat protoknot", consisting of an 8-residue cyclic segment followed by a 13-residue linear segment that loops back and threads through the cyclic segment. MccJ25 is the first example of a lariat protoknot involving a backbone-side chain amide linkage.

About this Structure

1PP5 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Structure of antibacterial peptide microcin J25: a 21-residue lariat protoknot., Bayro MJ, Mukhopadhyay J, Swapna GV, Huang JY, Ma LC, Sineva E, Dawson PE, Montelione GT, Ebright RH, J Am Chem Soc. 2003 Oct 15;125(41):12382-3. PMID:14531661

Page seeded by OCA on Sun Mar 30 23:02:38 2008

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