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1e9m
From Proteopedia
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FERREDOXIN VI FROM RHODOBACTER CAPSULATUS
Overview
A [2Fe-2S] ferredoxin found in the photosynthetic bacterium Rhodobacter, capsulatus has been purified in recombinant form from Escherichia coli., This protein, called FdVI, resembles ferredoxins involved in iron-sulfur, cluster biosynthesis in various prokaryotic and eukaryotic cells. Purified, recombinant FdVI was recovered in high yields and appeared to be, indistinguishable from the genuine R. capsulatus ferredoxin based on, UV-visible absorption and EPR spectroscopy and mass spectrometry. FdVI has, been crystallized in the oxidized state by a sitting-drop vapour-diffusion, technique using sodium formate as precipitant. Seeding larger drops from a, previous hanging-drop-grown small crystal resulted in the formation of, long red-brown prismatic needles. Preliminary X-ray diffraction analysis, indicated that FdVI crystals are orthorhombic and belong to the space, group P2(1)2(1)2(1), with unit-cell parameters a = 45.87, b = 49.83, c =, 54.29 A.
About this Structure
1E9M is a Single protein structure of sequence from Rhodobacter capsulatus with FES as ligand. Structure known Active Site: FES. Full crystallographic information is available from OCA.
Reference
Crystallization and preliminary X-ray diffraction analysis of a [2Fe-2S] ferredoxin (FdVI) from Rhodobacter capsulatus., Armengaud J, Sainz G, Jouanneau Y, Sieker LC, Acta Crystallogr D Biol Crystallogr. 2001 Feb;57(Pt 2):301-3. PMID:11173487[[Category: [2fe-2s]]]
Page seeded by OCA on Mon Nov 5 16:08:56 2007
