| Structural highlights
5j51 is a 8 chain structure with sequence from Artocarpus integer. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
| | Ligands: | , , , |
| Related: | 1ugw, 1jac, 1kuj, 1ws5, 1ws4, 1uh0, 1m26, 1ugx, 1uh1, 1ugy, 1ku8, 4r6n, 4r6o, 4r6p, 4r6q, 4r6r, 5j4x, 5j50, 5j52 |
| Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Function
[LECA_ARTIN] D-galactose-specific lectin, binds the T-antigen structure Gal-beta1,3-GalNAc (Thomsen-Friedenreich-antigen-specific lectin). Potent and selective stimulant of distinct T- and B-cell functions. Shows a unique ability to specifically recognize IgA-1 from human serum. [LECB3_ARTIN] D-galactose-specific lectin, binds the T-antigen structure Gal-beta1,3-GalNAc (Thomsen-Friedenreich-antigen-specific lectin). Potent and selective stimulant of distinct T- and B-cell functions. Shows a unique ability to specifically recognize IgA-1 from human serum.
Publication Abstract from PubMed
The crystal structures of jacalin complexed with Gal alpha-(1,4) Gal and Gal alpha-(1,3) Gal beta-(1,4) Gal have been determined with the primary objective of exploring the effect of linkage on the location of reducing and non-reducing sugars in the extended binding site of the lectin, an issue which has not been studied thoroughly. Contrary to the earlier surmise based on simple steric considerations, the two structures demonstrate that alpha-linked sugars can bind to jacalin with nonreducing sugar at the primary binding site. This is made possible substantially on account of the hitherto underestimated plasticity of a non-polar region of the extended binding site. Modeling studies involving conformational search and energy minimization, along with available crystallographic and thermodynamic data, indicate a strong preference for complexation with Gal beta-(1,3) Gal with the reducing Gal at the primary site, followed by that with Gal alpha-(1,3) Gal, with the reducing or non-reducing Gal located at the primary binding site. This observation is in consonance with the facility of jacalin to bind mucin type O-glycans containing T-antigen core. (c) 2016 IUBMB Life, 68(12):971-979, 2016.
Effect of linkage on the location of reducing and nonreducing sugars bound to jacalin.,Abhinav KV, Sharma K, Surolia A, Vijayan M IUBMB Life. 2016 Dec;68(12):971-979. doi: 10.1002/iub.1572. Epub 2016 Nov 3. PMID:27808459[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Abhinav KV, Sharma K, Surolia A, Vijayan M. Effect of linkage on the location of reducing and nonreducing sugars bound to jacalin. IUBMB Life. 2016 Dec;68(12):971-979. doi: 10.1002/iub.1572. Epub 2016 Nov 3. PMID:27808459 doi:http://dx.doi.org/10.1002/iub.1572
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