| Structural highlights
Function
[VM1LA_BOTLC] Non-hemorrhagic metalloproteinase that hydrolyzes the alpha chains of fibrinogen, as well as fibrin, fibronectin and casein. Beta and gamma chains are also hydrolyzed, but more slowly. Thrombolytic activity is also observed. Induces detachment of endothelial cells followed by death, and inhibits endothelial cell adhesion to fibronectin. Induces edema in mouse paw. Inhibits ADP-induced platelet aggregation on human platelet-rich plasma with an IC(50) of 2.8 uM.[1] [2]
Publication Abstract from PubMed
Leucurolysin-a (leuc-a) is a class P-I snake-venom metalloproteinase isolated from the venom of the South American snake Bothrops leucurus (white-tailed jararaca). The mature protein is composed of 202 amino-acid residues in a single polypeptide chain. It contains a blocked N-terminus and is not glycosylated. In vitro studies revealed that leuc-a dissolves clots made either from purified fibrinogen or from whole blood. Unlike some other venom fibrinolytic metalloproteinases, leuc-a has no haemorrhagic activity. Leuc-a was sequenced and was crystallized using the hanging-drop vapour-diffusion technique. Crystals were obtained using PEG 6000 or PEG 1500. Diffraction data to 1.80 and 1.60 A resolution were collected from two crystals (free enzyme and the endogenous ligand-protein complex, respectively). They both belonged to space group P2(1)2(1)2(1), with very similar unit-cell parameters (a = 44.0, b = 56.2, c = 76.3 A for the free-enzyme crystal).
Complete amino-acid sequence, crystallization and preliminary X-ray diffraction studies of leucurolysin-a, a nonhaemorrhagic metalloproteinase from Bothrops leucurus snake venom.,Ferreira RN, Rates B, Richardson M, Guimaraes BG, Sanchez EO, Pimenta AM, Nagem RA Acta Crystallogr Sect F Struct Biol Cryst Commun. 2009 Aug 1;65(Pt 8):798-801., doi: 10.1107/S1744309109025767. Epub 2009 Jul 25. PMID:19652343[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Bello CA, Hermogenes AL, Magalhaes A, Veiga SS, Gremski LH, Richardson M, Sanchez EF. Isolation and biochemical characterization of a fibrinolytic proteinase from Bothrops leucurus (white-tailed jararaca) snake venom. Biochimie. 2006 Feb;88(2):189-200. Epub 2005 Aug 19. PMID:16139412 doi:http://dx.doi.org/10.1016/j.biochi.2005.07.008
- ↑ Gremski LH, Chaim OM, Paludo KS, Sade YB, Otuki MF, Richardson M, Gremski W, Sanchez EF, Veiga SS. Cytotoxic, thrombolytic and edematogenic activities of leucurolysin-a, a metalloproteinase from Bothrops leucurus snake venom. Toxicon. 2007 Jul;50(1):120-34. Epub 2007 Mar 14. PMID:17482228 doi:http://dx.doi.org/10.1016/j.toxicon.2007.03.002
- ↑ Ferreira RN, Rates B, Richardson M, Guimaraes BG, Sanchez EO, Pimenta AM, Nagem RA. Complete amino-acid sequence, crystallization and preliminary X-ray diffraction studies of leucurolysin-a, a nonhaemorrhagic metalloproteinase from Bothrops leucurus snake venom. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2009 Aug 1;65(Pt 8):798-801., doi: 10.1107/S1744309109025767. Epub 2009 Jul 25. PMID:19652343 doi:http://dx.doi.org/10.1107/S1744309109025767
|
