Sandbox Reserved 1290
From Proteopedia
Human core-PIC in the initial transcribing state (no IIS)
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'Molecules it Interacts With and where '
The protein binds to GDP as well as the following ligands in order to promote the attachment of the protein complex to the ribosome A site.
PHOSHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER
PHENYLALANINE
MAGNESIUM ION
'Origin'
It has domains that are created in yeast (phenyl-transfer RNA) , in the heat resistant Thermus aquaticus (EF-Tu elongation factor, and can be synthetically manufactured.
'Structure'
It has 3 domains. G proteins, Elongation Factors, and the EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain. It is composed of 6 chains, which combine in alignment.
Specific are highlighted here. The ligands listed above, GDP, Phe, and Mg+2 ion each attach at these locations which are still being explored.
which play a crucial role in binding to the ribosome during translation. They form positive pockets with which negative amino acids can bind to.
'Molecules it Interacts With and where '
The protein binds to GDP as well as the following ligands in order to promote the attachment of the protein complex to the ribosome A site.
PHOSHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER
PHENYLALANINE
MAGNESIUM ION
'Origin'
It has domains that are created in yeast (phenyl-transfer RNA) , in the heat resistant Thermus aquaticus (EF-Tu elongation factor, and can be synthetically manufactured.
'Structure'
It has 3 domains. G proteins, Elongation Factors, and the EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain. It is composed of 6 chains, which combine in alignment.
Specific are highlighted here.
which play a crucial role in binding to the ribosome during translation.
'Function"
The protein complex participates in placing the amino acids in their correct order when messenger RNA is translated into a protein sequence on the ribosome by promoting GTP-dependent binding of tRNA to the A site of the ribosome. In other words, it is involved with elongation during polypeptide synthesis.
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Function
Human core-PIC helps in the transcription of DNA into using four ribonucleotide triphosphates as substrates. The molecule is a component of RNA Polymerase II, and thus is integral in the synthesis of mRNA precursors and functional non-coding RNA. Within the complex is the , which holds the TATA box binding protein- this polypeptide will bind to the TATA consensus sequence and help to initiate transcription of an mRNA transcript. The P chain is assisted by IF-2, located on the .
What it Interacts With
Human core-PIC interacts with DNA, ribonucleotide triphosphates, mRNA, and DNA Polymerase II.
Where is interacts
The molecule interacts with DNA in the nucleus, where the mRNA transcript is synthesized.
Where it originates
Human core-PIC originates in homo sapiens, or the modern day human.
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