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spinqualitylabelsall models 3D Representation of CzrA with Zn Bound Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Contents 1 CzrA 2 Biological Function 3 Structural Overview 4 DNA Bound State 5 Zinc Ligand(s) 6 Other Ligands 7 References

CzrA

Biological Function

Structural Overview

DNA Bound State

The have been found to occur at the Ser 54 and 57 along with His 58. These residues are likely to interact with the 5'-TGAA sequence found in the half-site of the DNA. These residues are found in the N terminal of the R helix. The residues involved in the are Val 42 and Gln 53. This was experimentally determined by replacing the Gln and Val with Ala residues and measuring the binding capacity; the Ka decresed by 11 and 160-fold respectively^[1] The previously mentioned Ser and His residues were found to bind the DNA with a similar affinity as the fully inhibited Zn²⁺.

Zinc Ligand(s)

Other Ligands

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References

Arunkumar A., Campanello G., Giedroc D. (2009). Solution Structure of a paradigm ArsR family zinc sensor in the DNA-bound state. PNAS 106:43 18177-18182