1u3e
From Proteopedia
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| , resolution 2.92Å | |||||||
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| Ligands: | , , , , , , , | ||||||
| Gene: | bacteriophage SP01 (Bacillus phage SPO1) | ||||||
| Related: | 1I3J, 1A73, 1FSJ, 1PT3
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
DNA binding and cleavage by the HNH homing endonuclease I-HmuI
Overview
The structure of I-HmuI, which represents the last family of homing endonucleases without a defining crystallographic structure, has been determined in complex with its DNA target. A series of diverse protein structural domains and motifs, contacting sequential stretches of nucleotide bases, are distributed along the DNA target. I-HmuI contains an N-terminal domain with a DNA-binding surface found in the I-PpoI homing endonuclease and an associated HNH/N active site found in the bacterial colicins, and a C-terminal DNA-binding domain previously observed in the I-TevI homing endonuclease. The combination and exchange of these features between protein families indicates that the genetic mobility associated with homing endonucleases extends to the level of independent structural domains. I-HmuI provides an unambiguous structural connection between the His-Cys box endonucleases and the bacterial colicins, supporting the hypothesis that these enzymes diverged from a common ancestral nuclease.
About this Structure
1U3E is a Single protein structure of sequence from Bacillus phage spo1. Full crystallographic information is available from OCA.
Reference
DNA binding and cleavage by the HNH homing endonuclease I-HmuI., Shen BW, Landthaler M, Shub DA, Stoddard BL, J Mol Biol. 2004 Sep 3;342(1):43-56. PMID:15313606
Page seeded by OCA on Mon Mar 31 00:04:55 2008
