Crystal structure of an exonuclease resistant RNA from Zika virus
Publication Abstract from PubMed
The outbreak of Zika virus (ZIKV) and associated fetal microcephaly mandates efforts to understand the molecular processes of infection. Related flaviviruses produce noncoding subgenomic flaviviral RNAs (sfRNAs) that are linked to pathogenicity in fetal mice. These viruses make sfRNAs by co-opting a cellular exonuclease via structured RNAs called xrRNAs. We found that ZIKV-infected monkey and human epithelial cells, mouse neurons, and mosquito cells produce sfRNAs. The RNA structure that is responsible for ZIKV sfRNA production forms a complex fold that is likely found in many pathogenic flaviviruses. Mutations that disrupt the structure affect exonuclease resistance in vitro and sfRNA formation during infection. The complete ZIKV xrRNA structure clarifies the mechanism of exonuclease resistance and identifies features that may modulate function in diverse flaviviruses.
Zika virus produces noncoding RNAs using a multi-pseudoknot structure that confounds a cellular exonuclease.,Akiyama BM, Laurence HM, Massey AR, Costantino DA, Xie X, Yang Y, Shi PY, Nix JC, Beckham JD, Kieft JS Science. 2016 Dec 2;354(6316):1148-1152. Epub 2016 Nov 10. PMID:27934765[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
The Basics
Click to represent the molecule by the DRuMS Color Scheme.
It shows the molecule is composed of RNA.
Click to see this simplified view where the RNA-RNA interactions are illustrated an the individual nucleotide bases are indicated by letters.
the RNA is colored to match the secondary structure in the paper.
TO DO: NEED AN IMAGE OF SECONDARY STRUCTURE DIAGRAM TO MATCH SCENE.
A .
In the crystal structure there is additionally observed and a bound.
5' interactions of the RNA highlighted in .
PDB Entry
Related Structures and Topics
Reference
- Akiyama BM, Laurence HM, Massey AR, Costantino DA, Xie X, Yang Y, Shi PY, Nix JC, Beckham JD, Kieft JS. Zika virus produces noncoding RNAs using a multi-pseudoknot structure that confounds a cellular exonuclease. Science. 2016 Dec 2;354(6316):1148-1152. Epub 2016 Nov 10. PMID:27934765 doi:http://dx.doi.org/10.1126/science.aah3963
