| Structural highlights
4ds7 is a 8 chain structure with sequence from Baker's yeast and Candida sphaerica. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
| | Ligands: | , , |
| Related: | 1lkj |
| Gene: | CMD1, KLLA0B09427g (Candida sphaerica), SPC110, NUF1, XCM1, YDR356W, D9476.3 (Baker's yeast) |
| Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Function
[CALM_KLULA] Calmodulin mediates the control of a large number of enzymes, ion channels and other proteins by Ca(2+). Among the enzymes to be stimulated by the calmodulin-Ca(2+) complex are a number of protein kinases and phosphatases. [SP110_YEAST] Component of the spindle pole body (SPB) required for the proper execution of spindle pole body (SPB) duplication. Potential role in cross-linking filaments or anchoring other molecules. It is essential for growth.[1] [2] [3] [4] [5] [6]
Publication Abstract from PubMed
Microtubule organizing centers (MTOCs) form, anchor and stabilize the polarized network of microtubules in a cell. The central MTOC is the centrosome that duplicates during the cell cycle and during mitosis assembles a bipolar spindle to capture and segregate sister chromatids. Yet, despite their importance in cell biology, the physical structure of MTOCs is poorly understood. Here we determine the molecular architecture of the core of the yeast spindle pole body (SPB) by Bayesian integrative structure modeling based on in vivo FRET, small-angle X-ray scattering (SAXS), X-ray crystallography, electron microscopy and two-hybrid analysis. The model is validated by several methods that include a genetic analysis of the conserved PACT domain that recruits Spc110, a protein related to pericentrin, to the SPB. The model suggests that calmodulin can act as a protein cross-linker and Spc29 is an extended, flexible protein. The model led to the identification of a single, essential heptad in the coiled coil of Spc110 and a minimal PACT domain. It also led to a proposed pathway for the integration of Spc110 into the SPB.
The molecular architecture of the yeast spindle pole body core determined by Bayesian integrative modeling.,Viswanath S, Bonomi M, Kim SJ, Klenchin VA, Taylor KC, Yabut KC, Umbreit NT, Van Epps HA, Meehl J, Jones MH, Russel D, Velazquez-Muriel JA, Winey M, Rayment I, Davis TN, Sali A, Muller EG Mol Biol Cell. 2017 Aug 16. pii: mbc.E17-06-0397. doi: 10.1091/mbc.E17-06-0397. PMID:28814505[7]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Elliott S, Knop M, Schlenstedt G, Schiebel E. Spc29p is a component of the Spc110p subcomplex and is essential for spindle pole body duplication. Proc Natl Acad Sci U S A. 1999 May 25;96(11):6205-10. PMID:10339566
- ↑ Friedman DB, Kern JW, Huneycutt BJ, Vinh DB, Crawford DK, Steiner E, Scheiltz D, Yates J 3rd, Resing KA, Ahn NG, Winey M, Davis TN. Yeast Mps1p phosphorylates the spindle pole component Spc110p in the N-terminal domain. J Biol Chem. 2001 May 25;276(21):17958-67. Epub 2001 Mar 6. PMID:11278681 doi:http://dx.doi.org/10.1074/jbc.M010461200
- ↑ Mirzayan C, Copeland CS, Snyder M. The NUF1 gene encodes an essential coiled-coil related protein that is a potential component of the yeast nucleoskeleton. J Cell Biol. 1992 Mar;116(6):1319-32. PMID:1541631
- ↑ Huisman SM, Smeets MF, Segal M. Phosphorylation of Spc110p by Cdc28p-Clb5p kinase contributes to correct spindle morphogenesis in S. cerevisiae. J Cell Sci. 2007 Feb 1;120(Pt 3):435-46. Epub 2007 Jan 9. PMID:17213332 doi:http://dx.doi.org/10.1242/jcs.03342
- ↑ Kilmartin JV, Dyos SL, Kershaw D, Finch JT. A spacer protein in the Saccharomyces cerevisiae spindle poly body whose transcript is cell cycle-regulated. J Cell Biol. 1993 Dec;123(5):1175-84. PMID:7503995
- ↑ Knop M, Schiebel E. Spc98p and Spc97p of the yeast gamma-tubulin complex mediate binding to the spindle pole body via their interaction with Spc110p. EMBO J. 1997 Dec 1;16(23):6985-95. PMID:9384578 doi:http://dx.doi.org/10.1093/emboj/16.23.6985
- ↑ Viswanath S, Bonomi M, Kim SJ, Klenchin VA, Taylor KC, Yabut KC, Umbreit NT, Van Epps HA, Meehl J, Jones MH, Russel D, Velazquez-Muriel JA, Winey M, Rayment I, Davis TN, Sali A, Muller EG. The molecular architecture of the yeast spindle pole body core determined by Bayesian integrative modeling. Mol Biol Cell. 2017 Aug 16. pii: mbc.E17-06-0397. doi: 10.1091/mbc.E17-06-0397. PMID:28814505 doi:http://dx.doi.org/10.1091/mbc.E17-06-0397
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