1zuy

Structural highlights

Function

[MYO5_YEAST] One of two redundant type-I myosins implicated in the organization of the actin cytoskeleton. Required for proper actin cytoskeleton polarization and for the internalization step in endocytosis. At the cell cortex, assembles in patch-like structures together with proteins from the actin-polymerizing machinery and promotes actin assembly. Functions redundantly with LAS17 as actin nucleation-promoting factor (NPF) for the Arp2/3 complex. Motor domain phosphorylation by PAK kinases CLA4 and STE20 promotes CDC42-regulated actin assembly. Functions together with the NPF PAN1 in late stages of endocytosis. Motor domain phosphorylation by PDK1 kinases PKH1 and PKH2, and by SGK kinases YPK1 and YPK2, promotes ligand-induced, but not constitutive endocytosis of the G protein-coupled receptor STE2.^{[1] [2] [3] [4] [5] [6] [7] [8] [9]}

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

References

1. ↑ Goodson HV, Anderson BL, Warrick HM, Pon LA, Spudich JA. Synthetic lethality screen identifies a novel yeast myosin I gene (MYO5): myosin I proteins are required for polarization of the actin cytoskeleton. J Cell Biol. 1996 Jun;133(6):1277-91. PMID:8682864
2. ↑ Geli MI, Riezman H. Role of type I myosins in receptor-mediated endocytosis in yeast. Science. 1996 Apr 26;272(5261):533-5. PMID:8614799
3. ↑ Anderson BL, Boldogh I, Evangelista M, Boone C, Greene LA, Pon LA. The Src homology domain 3 (SH3) of a yeast type I myosin, Myo5p, binds to verprolin and is required for targeting to sites of actin polarization. J Cell Biol. 1998 Jun 15;141(6):1357-70. PMID:9628892
4. ↑ Geli MI, Lombardi R, Schmelzl B, Riezman H. An intact SH3 domain is required for myosin I-induced actin polymerization. EMBO J. 2000 Aug 15;19(16):4281-91. PMID:10944111 doi:http://dx.doi.org/10.1093/emboj/19.16.4281
5. ↑ Evangelista M, Klebl BM, Tong AH, Webb BA, Leeuw T, Leberer E, Whiteway M, Thomas DY, Boone C. A role for myosin-I in actin assembly through interactions with Vrp1p, Bee1p, and the Arp2/3 complex. J Cell Biol. 2000 Jan 24;148(2):353-62. PMID:10648568
6. ↑ Lechler T, Shevchenko A, Li R. Direct involvement of yeast type I myosins in Cdc42-dependent actin polymerization. J Cell Biol. 2000 Jan 24;148(2):363-73. PMID:10648569
7. ↑ Wesche S, Arnold M, Jansen RP. The UCS domain protein She4p binds to myosin motor domains and is essential for class I and class V myosin function. Curr Biol. 2003 Apr 29;13(9):715-24. PMID:12725728
8. ↑ Toi H, Fujimura-Kamada K, Irie K, Takai Y, Todo S, Tanaka K. She4p/Dim1p interacts with the motor domain of unconventional myosins in the budding yeast, Saccharomyces cerevisiae. Mol Biol Cell. 2003 Jun;14(6):2237-49. Epub 2003 Feb 6. PMID:12808026 doi:http://dx.doi.org/10.1091/mbc.E02-09-0616
9. ↑ Grosshans BL, Grotsch H, Mukhopadhyay D, Fernandez IM, Pfannstiel J, Idrissi FZ, Lechner J, Riezman H, Geli MI. TEDS site phosphorylation of the yeast myosins I is required for ligand-induced but not for constitutive endocytosis of the G protein-coupled receptor Ste2p. J Biol Chem. 2006 Apr 21;281(16):11104-14. Epub 2006 Feb 13. PMID:16478726 doi:http://dx.doi.org/10.1074/jbc.M508933200