Structural highlights
Function
[YYCF_BACSU] Member of the two-component regulatory system YycG/YycF involved in the regulation of the ftsAZ operon, the yocH, ykvT, cwlO, lytE, ydjM, yjeA, yoeB genes and the tagAB and tagDEF operons. Probably phosphorylates YycF. Binds to the ftsAZ P1 promoter sequence in vitro. YycF has been shown to directly bind to the regulatory regions of yocH, ykvT, tagAB/tagDEF. Activates cwlO, lytE and ydjM and represses yoeB and yjeA.[1] [2] [3]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
A response regulator YycF and its cognate sensor kinase YycG constitute the two-component signal transduction system essential for growth of Gram-positive bacteria with a low GC content. We have determined the X-ray crystal structure of the effector domain of Bacillus subtilis YycF involved in DNA binding. The structure, containing a winged helix-turn-helix motif, was found to be very similar to that of the response regulator PhoB from Escherichia coli. Specific binding of YycF to the PhoB-regulated alkaline phosphatase promoter was also demonstrated.
Response regulator YycF essential for bacterial growth: X-ray crystal structure of the DNA-binding domain and its PhoB-like DNA recognition motif.,Okajima T, Doi A, Okada A, Gotoh Y, Tanizawa K, Utsumi R FEBS Lett. 2008 Oct 15;582(23-24):3434-8. Epub 2008 Sep 26. PMID:18789936[4]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Fabret C, Hoch JA. A two-component signal transduction system essential for growth of Bacillus subtilis: implications for anti-infective therapy. J Bacteriol. 1998 Dec;180(23):6375-83. PMID:9829949
- ↑ Fukuchi K, Kasahara Y, Asai K, Kobayashi K, Moriya S, Ogasawara N. The essential two-component regulatory system encoded by yycF and yycG modulates expression of the ftsAZ operon in Bacillus subtilis. Microbiology. 2000 Jul;146 ( Pt 7):1573-83. PMID:10878122
- ↑ Bisicchia P, Noone D, Lioliou E, Howell A, Quigley S, Jensen T, Jarmer H, Devine KM. The essential YycFG two-component system controls cell wall metabolism in Bacillus subtilis. Mol Microbiol. 2007 Jul;65(1):180-200. PMID:17581128 doi:http://dx.doi.org/MMI5782
- ↑ Okajima T, Doi A, Okada A, Gotoh Y, Tanizawa K, Utsumi R. Response regulator YycF essential for bacterial growth: X-ray crystal structure of the DNA-binding domain and its PhoB-like DNA recognition motif. FEBS Lett. 2008 Oct 15;582(23-24):3434-8. Epub 2008 Sep 26. PMID:18789936 doi:10.1016/j.febslet.2008.09.007
