1oad

From Proteopedia

proteopedia linkproteopedia link

spinqualitylabelsall models 1oad, resolution 1.5Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

GLUCOSE ISOMERASE FROM STREPTOMYCES RUBIGINOSUS IN P21212 CRYSTAL FORM

Overview

Glucose isomerase from Streptomyces rubiginosus was crystallized in two, forms: I222, with one molecule of 44 kDa in the asymmetric unit, and, P2(1)2(1)2, with two unique molecules. The I222 structure is known, but, the P2(1)2(1)2 form has not been solved before. X-ray diffraction data for, the P2(1)2(1)2 form were collected at a wavelength of 1.54 A and data for, the I222 form were collected at three different wavelengths: 1.34, 1.07, and 0.98 A. The amount of anomalous signal from one Mn and eight S atoms, in these data sets varies from 1.24% to as low as 0.56%. The dual-space, direct-methods program SHELXD, run against the Bijvoet differences, gave a, clear solution of all anomalous scatterers for all data sets. The Mn, positions only were used for SAD phasing of all four data sets. The, electron-density map after density modification, resulting from the, phasing of a single-wavelength data set and based purely on the anomalous, deltaf" contribution, was clearly interpretable; an almost complete model, of the protein was built by wARP without human intervention in all four, cases. As far as is known, this is the first time that an anomalous signal, as low as 0.6% has successfully been used to determine the structure of a, macromolecule.

About this Structure

1OAD is a Single protein structure of sequence from Streptomyces rubiginosus with MN, MG, MRD, MQD, TRS and MPD as ligands. Active as Xylose isomerase, with EC number 5.3.1.5 Structure known Active Site: AC1. Full crystallographic information is available from OCA.

Reference

SAD manganese in two crystal forms of glucose isomerase., Ramagopal UA, Dauter M, Dauter Z, Acta Crystallogr D Biol Crystallogr. 2003 May;59(Pt 5):868-75. Epub 2003, Apr 25. PMID:12777803

Page seeded by OCA on Mon Nov 5 16:48:11 2007