Structural highlights
5k1x is a 1 chain structure with sequence from Dsm 2053. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
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| Ligands: | , , , , |
| Related: | 5k1p |
| Gene: | pylS, MM_1445 (DSM 2053) |
| Activity: | Pyrrolysine--tRNA(Pyl) ligase, with EC number 6.1.1.26 |
| Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Function
[PYLS_METMA] Catalyzes the attachment of pyrrolysine to tRNA(Pyl). Pyrrolysine is a lysine derivative encoded by the termination codon UAG (By similarity).
Publication Abstract from PubMed
Fluorophenylalanines bearing 2-5 fluorine atoms at the phenyl ring have been genetically encoded by amber codon. Replacement of F59, a phenylalanine residue that is directly involved in interactions with trimethylated K9 of histone H3, in the Mpp8 chromodomain recombinantly with fluorophenylalanines significantly impairs the binding to a K9-trimethylated H3 peptide.
Genetically encoded fluorophenylalanines enable insights into the recognition of lysine trimethylation by an epigenetic reader.,Lee YJ, Schmidt MJ, Tharp JM, Weber A, Koenig AL, Zheng H, Gao J, Waters ML, Summerer D, Liu WR Chem Commun (Camb). 2016 Oct 18;52(85):12606-12609. PMID:27711380[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Lee YJ, Schmidt MJ, Tharp JM, Weber A, Koenig AL, Zheng H, Gao J, Waters ML, Summerer D, Liu WR. Genetically encoded fluorophenylalanines enable insights into the recognition of lysine trimethylation by an epigenetic reader. Chem Commun (Camb). 2016 Oct 18;52(85):12606-12609. PMID:27711380 doi:http://dx.doi.org/10.1039/c6cc05959g
