Structural highlights
3o16 is a 1 chain structure with sequence from "vibrio_subtilis"_ehrenberg_1835 "vibrio subtilis" ehrenberg 1835. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
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| Related: | 3o15 |
| Gene: | BSU38290, ipa-26d, thiC, thiE, ywbK ("Vibrio subtilis" Ehrenberg 1835) |
| Activity: | Thiamine-phosphate diphosphorylase, with EC number 2.5.1.3 |
| Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Function
[THIE_BACSU] Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP). Is also able to use the 2-methoxy analog MeO-HMP-PP, as substrate in vitro, but not the 2-trifluoromethyl analog CF(3)-HMP-PP.[1] [2]
References
- ↑ Zhang Y, Taylor SV, Chiu HJ, Begley TP. Characterization of the Bacillus subtilis thiC operon involved in thiamine biosynthesis. J Bacteriol. 1997 May;179(9):3030-5. PMID:9139923
- ↑ Reddick JJ, Nicewonger R, Begley TP. Mechanistic studies on thiamin phosphate synthase: evidence for a dissociative mechanism. Biochemistry. 2001 Aug 28;40(34):10095-102. PMID:11513588
