Structural highlights
5oxj is a 3 chain structure with sequence from Atcc 25104. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
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| Ligands: | , , , |
| NonStd Res: | , |
| Gene: | polA, pol1 (ATCC 25104) |
| Activity: | DNA-directed DNA polymerase, with EC number 2.7.7.7 |
| Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Publication Abstract from PubMed
The possibility to sequence cytotoxic O(6)-alkylG DNA adducts would greatly benefit research. Recently we reported a benzimidazole-derived nucleotide that is selectively incorporated opposite the damaged site by a mutated DNA polymerase. Here we provide the structural basis for this reaction which may spur future developments in DNA damage sequencing.
Structural basis for the selective incorporation of an artificial nucleotide opposite a DNA adduct by a DNA polymerase.,Betz K, Nilforoushan A, Wyss LA, Diederichs K, Sturla SJ, Marx A Chem Commun (Camb). 2017 Nov 23;53(94):12704-12707. doi: 10.1039/c7cc07173f. PMID:29136072[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Betz K, Nilforoushan A, Wyss LA, Diederichs K, Sturla SJ, Marx A. Structural basis for the selective incorporation of an artificial nucleotide opposite a DNA adduct by a DNA polymerase. Chem Commun (Camb). 2017 Nov 23;53(94):12704-12707. doi: 10.1039/c7cc07173f. PMID:29136072 doi:http://dx.doi.org/10.1039/c7cc07173f
