| Structural highlights
Function
[MORF2_ARATH] Involved in plastid rRNA processing and consequently in translation and early chloroplast differentiation (PubMed:12678554). Involved in organellar RNA editing. Required for the processing of multiple editing sites in plastids (PubMed:22411807, PubMed:23818871).[1] [2] [3]
Publication Abstract from PubMed
RNA editing is a post-transcription process that alters the genetic information on RNA molecules. In plastids and mitochondria of flowering plants, the multiple organellar RNA editing factors (MORFs) interact with the PLS-type pentatricopeptide repeat (PPR) proteins and participate in RNA editing of cytidine-to-uridine conversion. The PPR proteins recognize cytidine targets around the editing sites, and the MORF proteins modulate the RNA-binding activity of the PPR proteins. Here, we report the structure of the Arabidopsis thaliana chloroplast MORF2 at 2.4 A resolution. The structure, adopting typical MORF-box fold as observed in mitochondrial MORF1 and chloroplast MORF9, reveals an MORF1-like dimerization mode. The difference between the two dimerization modes can be attributed to F157 (corresponding F162 in MORF1 and W160 in MORF9), which causes a 60 degrees shift upon dimerization. This observation, together with the PPR-MORF2 model, suggests a dimer-to-monomer transition during RNA editosome formation.
Crystal structure of the chloroplast RNA editing factor MORF2.,Yang J, Zhang M, Wang X Biochem Biophys Res Commun. 2017 Dec 8. pii: S0006-291X(17)32435-X. doi:, 10.1016/j.bbrc.2017.12.044. PMID:29229384[4]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Bisanz C, Begot L, Carol P, Perez P, Bligny M, Pesey H, Gallois JL, Lerbs-Mache S, Mache R. The Arabidopsis nuclear DAL gene encodes a chloroplast protein which is required for the maturation of the plastid ribosomal RNAs and is essential for chloroplast differentiation. Plant Mol Biol. 2003 Mar;51(5):651-63. PMID:12678554
- ↑ Takenaka M, Zehrmann A, Verbitskiy D, Kugelmann M, Hartel B, Brennicke A. Multiple organellar RNA editing factor (MORF) family proteins are required for RNA editing in mitochondria and plastids of plants. Proc Natl Acad Sci U S A. 2012 Mar 27;109(13):5104-9. doi:, 10.1073/pnas.1202452109. Epub 2012 Mar 12. PMID:22411807 doi:http://dx.doi.org/10.1073/pnas.1202452109
- ↑ Bentolila S, Oh J, Hanson MR, Bukowski R. Comprehensive high-resolution analysis of the role of an Arabidopsis gene family in RNA editing. PLoS Genet. 2013 Jun;9(6):e1003584. doi: 10.1371/journal.pgen.1003584. Epub 2013 , Jun 20. PMID:23818871 doi:http://dx.doi.org/10.1371/journal.pgen.1003584
- ↑ Yang J, Zhang M, Wang X. Crystal structure of the chloroplast RNA editing factor MORF2. Biochem Biophys Res Commun. 2017 Dec 8. pii: S0006-291X(17)32435-X. doi:, 10.1016/j.bbrc.2017.12.044. PMID:29229384 doi:http://dx.doi.org/10.1016/j.bbrc.2017.12.044
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