Structural highlights
3tm7 is a 4 chain structure with sequence from Ecoli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
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| Ligands: | |
| Related: | 1aw8, 1ppy, 1pqe, 1pqf, 1pqh, 1pyq |
| Gene: | b0131, JW0127, panD (ECOLI) |
| Activity: | Aspartate 1-decarboxylase, with EC number 4.1.1.11 |
| Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Function
[PAND_ECOLI] Catalyzes the pyruvoyl-dependent decarboxylation of aspartate to produce beta-alanine.[1]
Publication Abstract from PubMed
The crystal structure of the Asn72Ala site-directed mutant of Escherichia coli aspartate alpha-decarboxylase (ADC) has been determined at 1.7 A resolution. The refined structure is consistent with the presence of a hydrolysis product serine in the active site in place of the pyruvoyl group required for catalysis, which suggests that the role of Asn72 is to protect the ester formed during ADC activation from hydrolysis. In previously determined structures of activated ADC, including the wild type and other site-directed mutants, the C-terminal region of the protein is disordered, but in the Asn72Ala mutant these residues are ordered owing to an interaction with the active site of the neighbouring symmetry-related multimer.
Structure of Escherichia coli aspartate alpha-decarboxylase Asn72Ala: probing the role of Asn72 in pyruvoyl cofactor formation.,Webb ME, Lobley CM, Soliman F, Kilkenny ML, Smith AG, Blundell TL, Abell C Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Apr 1;68(Pt 4):414-7. Epub, 2012 Mar 28. PMID:22505409[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Cronan JE Jr. Beta-alanine synthesis in Escherichia coli. J Bacteriol. 1980 Mar;141(3):1291-7. PMID:6767707
- ↑ Webb ME, Lobley CM, Soliman F, Kilkenny ML, Smith AG, Blundell TL, Abell C. Structure of Escherichia coli aspartate alpha-decarboxylase Asn72Ala: probing the role of Asn72 in pyruvoyl cofactor formation. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Apr 1;68(Pt 4):414-7. Epub, 2012 Mar 28. PMID:22505409 doi:10.1107/S1744309112009487
