Structural highlights
Function
[C1IFD2_ECOLX] RNA chaperone that binds small regulatory RNA (sRNAs) and mRNAs to facilitate mRNA translational regulation in response to envelope stress, environmental stress and changes in metabolite concentrations. Also binds with high specificity to tRNAs.[HAMAP-Rule:MF_00436]
Publication Abstract from PubMed
In bacteria, small RNAs (sRNAs) silence or activate target genes through base pairing with the mRNA, thereby modulating its translation. A central player in this process is the RNA chaperone Hfq, which facilitates the annealing of sRNAs with their target mRNAs. Hfq has two RNA-binding surfaces that recognize A-rich and U-rich sequences, and is believed to bind an sRNA-mRNA pair simultaneously. However, how Hfq promotes annealing remains unclear. Here, the crystal structure of Escherichia coli Hfq is presented in complex with U6-RNA bound to its proximal binding site at 0.97 A resolution, revealing the Hfq-RNA interaction in exceptional detail.
Structure of an Escherichia coli Hfq:RNA complex at 0.97 A resolution.,Schulz EC, Barabas O Acta Crystallogr F Struct Biol Commun. 2014 Nov;70(Pt 11):1492-7. doi:, 10.1107/S2053230X14020044. Epub 2014 Oct 31. PMID:25372815[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Schulz EC, Barabas O. Structure of an Escherichia coli Hfq:RNA complex at 0.97 A resolution. Acta Crystallogr F Struct Biol Commun. 2014 Nov;70(Pt 11):1492-7. doi:, 10.1107/S2053230X14020044. Epub 2014 Oct 31. PMID:25372815 doi:http://dx.doi.org/10.1107/S2053230X14020044
