1w3f
From Proteopedia
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CRYSTAL STRUCTURE OF THE HEMOLYTIC LECTIN FROM THE MUSHROOM LAETIPORUS SULPHUREUS COMPLEXED WITH N-ACETYLLACTOSAMINE IN THE GAMMA MOTIF
Overview
LSL is a lectin produced by the parasitic mushroom Laetiporus sulphureus, which exhibits hemolytic and hemagglutinating activities. Here, we report, the crystal structure of LSL refined to 2.6-A resolution determined by the, single isomorphous replacement method with the anomalous scatter (SIRAS), signal of a platinum derivative. The structure reveals that LSL is, hexameric, which was also shown by analytical ultracentrifugation. The, monomeric protein (35 kDa) consists of two distinct modules: an N-terminal, lectin module and a pore-forming module. The lectin module has a, beta-trefoil scaffold that bears structural similarities to those present, in toxins known to interact with galactose-related carbohydrates such as, the hemagglutinin component (HA1) of the progenitor toxin from Clostridium, botulinum, abrin, and ricin. On the other hand, the C-terminal, pore-forming module (composed of domains 2 and 3) exhibits, three-dimensional structural resemblances with domains 3 and 4 of the, beta-pore-forming toxin aerolysin from the Gram-negative bacterium, Aeromonas hydrophila, and domains 2 and 3 from the epsilon-toxin from, Clostridium perfringens. This finding reveals the existence of common, structural elements within the aerolysin-like family of toxins that could, be directly involved in membrane-pore formation. The crystal structures of, the complexes of LSL with lactose and N-acetyllactosamine reveal two, dissacharide-binding sites per subunit and permits the identification of, critical residues involved in sugar binding.
About this Structure
1W3F is a Single protein structure of sequence from Laetiporus sulphureus with NLC and GOL as ligands. Structure known Active Site: AC1. Full crystallographic information is available from OCA.
Reference
Structural analysis of the Laetiporus sulphureus hemolytic pore-forming lectin in complex with sugars., Mancheno JM, Tateno H, Goldstein IJ, Martinez-Ripoll M, Hermoso JA, J Biol Chem. 2005 Apr 29;280(17):17251-9. Epub 2005 Feb 1. PMID:15687495
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