2idc
From Proteopedia
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| , resolution 2.20Å | |||||||
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| Gene: | ASF1 and HHT1, SIN2 (Saccharomyces cerevisiae) | ||||||
| Related: | 1ROC
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structure of the Histone H3-Asf1 Chaperone Interaction
Overview
BACKGROUND: The histone H3/H4 chaperone Asf1 (anti-silencing function 1) is required for the establishment and maintenance of proper chromatin structure, as well as for genome stability in eukaryotes. Asf1 participates in both DNA replication-coupled (RC) and replication-independent (RI) histone deposition reactions in vitro and interacts with complexes responsible for both pathways in vivo. Asf1 is known to directly bind histone H3, however, high-resolution structural information about the geometry of this interaction was previously unknown. RESULTS: Here we report the structure of a histone/histone chaperone interaction. We have solved the 2.2 A crystal structure of the conserved N-terminal immunoglobulin fold domain of yeast Asf1 (residues 2-155) bound to the C-terminal helix of yeast histone H3 (residues 121-134). The structure defines a histone-binding patch on Asf1 consisting of both conserved and yeast-specific residues; mutation of these residues abrogates H3/H4 binding affinity. The geometry of the interaction indicates that Asf1 binds to histones H3/H4 in a manner that likely blocks sterically the H3/H3 interface of the nucleosomal four-helix bundle. CONCLUSION: These data clarify how Asf1 regulates histone stoichiometry to modulate epigenetic inheritance. The structure further suggests a physical model in which Asf1 contributes to interpretation of a "histone H3 barcode" for sorting H3 isoforms into different deposition pathways.
About this Structure
2IDC is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
Structure of the yeast histone H3-ASF1 interaction: implications for chaperone mechanism, species-specific interactions, and epigenetics., Antczak AJ, Tsubota T, Kaufman PD, Berger JM, BMC Struct Biol. 2006 Dec 13;6:26. PMID:17166288
Page seeded by OCA on Mon Mar 31 03:42:46 2008
Categories: Saccharomyces cerevisiae | Single protein | Antczak, A J. | Berger, J M. | Kaufman, P D. | Tsubota, T. | Asf1 | Chaperone | Chromatin | H3 | Histone | Ig-like fold
