Structural highlights
Function
[3XYN_ALCSP] Catalyzes the hydrolysis of beta-1,3-xylan into oligosaccharides, mainly xylotriose and xylobiose with smaller amounts of xylotetraose, xylose, xylopentaose and xylohexaose. Does not hydrolyze xylobiose, p-nitrophenyl-beta-xyloside, beta-1,4-xylan, carboxymethylcellulose, curdlan, glucomannan or beta-1,4-mannan.[1] [2]
Publication Abstract from PubMed
Here, we present the crystal structure of the family 31 carbohydrate-binding module (CBM) of beta-1,3-xylanase from Alcaligenes sp. strain XY-234 (AlcCBM31) determined at a resolution of 1.25A. The AlcCBM31 shows affinity with only beta-1,3-xylan. The AlcCBM31 molecule makes a beta-sandwich structure composed of eight beta-strands with a typical immunoglobulin fold and contains two intra-molecular disulfide bonds. The folding topology of AlcCBM31 differs from that of the large majority of other CBMs, in which eight beta-strands comprise a beta-sandwich structure with a typical jelly-roll fold. AlcCBM31 shows structural similarity with CBM structures of family 34 and family 9, which also adopt structures based on immunoglobulin folds.
The first crystal structure of a family 31 carbohydrate-binding module with affinity to beta-1,3-xylan.,Hashimoto H, Tamai Y, Okazaki F, Tamaru Y, Shimizu T, Araki T, Sato M FEBS Lett. 2005 Aug 15;579(20):4324-8. PMID:16061225[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Okazaki F, Tamaru Y, Hashikawa S, Li YT, Araki T. Novel carbohydrate-binding module of beta-1,3-xylanase from a marine bacterium, Alcaligenes sp. strain XY-234. J Bacteriol. 2002 May;184(9):2399-403. PMID:11948152
- ↑ Araki T, Inoue N, Morishita T. Purification and characterization of beta-1,3-xylanase from a marine bacterium, Alcaligenes sp. XY-234. J Gen Appl Microbiol. 1998 Aug;44(4):269-274. PMID:12501421
- ↑ Hashimoto H, Tamai Y, Okazaki F, Tamaru Y, Shimizu T, Araki T, Sato M. The first crystal structure of a family 31 carbohydrate-binding module with affinity to beta-1,3-xylan. FEBS Lett. 2005 Aug 15;579(20):4324-8. PMID:16061225 doi:http://dx.doi.org/S0014-5793(05)00820-3
