5o0y

Structural highlights

Function

[TLK2_HUMAN] Serine/threonine-protein kinase involved in the process of chromatin assembly and probably also DNA replication, transcription, repair, and chromosome segregation. Phosphorylates the chromatin assembly factors ASF1A AND ASF1B. Phosphorylation of ASF1A prevents its proteasome-mediated degradation, thereby enhancing chromatin assembly. Negative regulator of amino acid starvation-induced autophagy.^{[1] [2] [3] [4] [5] [6] [7]}

References

1. ↑ Sillje HH, Takahashi K, Tanaka K, Van Houwe G, Nigg EA. Mammalian homologues of the plant Tousled gene code for cell-cycle-regulated kinases with maximal activities linked to ongoing DNA replication. EMBO J. 1999 Oct 15;18(20):5691-702. PMID:10523312 doi:http://dx.doi.org/10.1093/emboj/18.20.5691
2. ↑ Sillje HH, Nigg EA. Identification of human Asf1 chromatin assembly factors as substrates of Tousled-like kinases. Curr Biol. 2001 Jul 10;11(13):1068-73. PMID:11470414
3. ↑ Groth A, Lukas J, Nigg EA, Sillje HH, Wernstedt C, Bartek J, Hansen K. Human Tousled like kinases are targeted by an ATM- and Chk1-dependent DNA damage checkpoint. EMBO J. 2003 Apr 1;22(7):1676-87. PMID:12660173 doi:10.1093/emboj/cdg151
4. ↑ Krause DR, Jonnalagadda JC, Gatei MH, Sillje HH, Zhou BB, Nigg EA, Khanna K. Suppression of Tousled-like kinase activity after DNA damage or replication block requires ATM, NBS1 and Chk1. Oncogene. 2003 Sep 4;22(38):5927-37. doi: 10.1038/sj.onc.1206691. PMID:12955071 doi:http://dx.doi.org/10.1038/sj.onc.1206691
5. ↑ Pilyugin M, Demmers J, Verrijzer CP, Karch F, Moshkin YM. Phosphorylation-mediated control of histone chaperone ASF1 levels by Tousled-like kinases. PLoS One. 2009 Dec 16;4(12):e8328. doi: 10.1371/journal.pone.0008328. PMID:20016786 doi:http://dx.doi.org/10.1371/journal.pone.0008328
6. ↑ McKnight NC, Jefferies HB, Alemu EA, Saunders RE, Howell M, Johansen T, Tooze SA. Genome-wide siRNA screen reveals amino acid starvation-induced autophagy requires SCOC and WAC. EMBO J. 2012 Apr 18;31(8):1931-46. doi: 10.1038/emboj.2012.36. Epub 2012 Feb 21. PMID:22354037 doi:10.1038/emboj.2012.36
7. ↑ Yamakawa A, Kameoka Y, Hashimoto K, Yoshitake Y, Nishikawa K, Tanihara K, Date T. cDNA cloning and chromosomal mapping of genes encoding novel protein kinases termed PKU-alpha and PKU-beta, which have nuclear localization signal. Gene. 1997 Nov 20;202(1-2):193-201. PMID:9427565