1wcm

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spinqualitylabelsall models 1wcm, resolution 3.8Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

COMPLETE 12-SUBUNIT RNA POLYMERASE II AT 3.8 ANG

Overview

We determined the x-ray structure of the RNA polymerase (Pol) II, subcomplex Rpb4/7 at 2.3 A resolution, combined it with a previous, structure of the 10-subunit polymerase core, and refined an atomic model, of the complete 12-subunit Pol II at 3.8-A resolution. Comparison of the, complete Pol II structure with structures of the Pol II core and free, Rpb4/7 shows that the core-Rpb4/7 interaction goes along with formation of, an alpha-helix in the linker region of the largest Pol II subunit and with, folding of the conserved Rpb7 tip loop. Details of the core-Rpb4/7, interface explain facilitated Rpb4/7 dissociation in a, temperature-sensitive Pol II mutant and specific assembly of Pol I with, its Rpb4/7 counterpart, A43/14. The refined atomic model of Pol II serves, as the new reference structure for analysis of the transcription mechanism, and enables structure solution of complexes of the complete enzyme with, additional factors and nucleic acids by molecular replacement.

About this Structure

1WCM is a Protein complex structure of sequences from Saccharomyces cerevisiae with ZN and MG as ligands. Active as DNA-directed RNA polymerase, with EC number 2.7.7.6 Structure known Active Site: AC1. Full crystallographic information is available from OCA.

Reference

Structures of complete RNA polymerase II and its subcomplex, Rpb4/7., Armache KJ, Mitterweger S, Meinhart A, Cramer P, J Biol Chem. 2005 Feb 25;280(8):7131-4. Epub 2004 Dec 9. PMID:15591044

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