2jm3
From Proteopedia
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| Ligands: | |||||||
| Gene: | F49E10.5 (Caenorhabditis elegans) | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Solution structure of the THAP domain from C. elegans C-terminal binding protein (CtBP)
Overview
The THAP (Thanatos-associated protein) domain is a recently discovered zinc-binding domain found in proteins involved in transcriptional regulation, cell-cycle control, apoptosis and chromatin modification. It contains a single zinc atom ligated by cysteine and histidine residues within a Cys-X(2-4)-Cys-X(35-53)-Cys-X(2)-His consensus. We have determined the NMR solution structure of the THAP domain from Caenorhabditis elegans C-terminal binding protein (CtBP) and show that it adopts a fold containing a treble clef motif, bearing similarity to the zinc finger-associated domain (ZAD) from Drosophila Grauzone. The CtBP THAP domain contains a large, positively charged surface patch and we demonstrate that this domain can bind to double-stranded DNA in an electrophoretic mobility-shift assay. These data, together with existing reports, indicate that THAP domains might exhibit a functional diversity similar to that observed for classical and GATA-type zinc fingers.
About this Structure
2JM3 is a Single protein structure of sequence from Caenorhabditis elegans. Full crystallographic information is available from OCA.
Reference
Solution structure of the THAP domain from Caenorhabditis elegans C-terminal binding protein (CtBP)., Liew CK, Crossley M, Mackay JP, Nicholas HR, J Mol Biol. 2007 Feb 16;366(2):382-90. Epub 2006 Nov 18. PMID:17174978
Page seeded by OCA on Mon Mar 31 03:59:04 2008
