2k0b

From Proteopedia

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spinqualitylabelsall models PDB ID 2k0b Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image
Gene:	SQSTM1, ORCA, OSIL (Homo sapiens)
Resources:	FirstGlance, OCA, PDBsum, RCSB
Coordinates:	save as pdb, mmCIF, xml

NMR structure of the UBA domain of p62 (SQSTM1)

Overview

The ubiquitin associated domain of p62 is a small three-helix bundle of approximately 50 residues that mediates the recognition of polyubiquitin chains and ubiquitylated substrates. The solution structure of a 52 residue construct containing this domain has been characterized using heteronuclear nuclear magnetic resonance (NMR) methods. The resulting ensemble of NMR-derived structures was used in molecular dynamics (MD) simulations to investigate the equilibrium conformation and dynamics of this domain. NOE and (15)N relaxation data have been used to validate the structural ensemble produced by the MD simulations and show a good correlation for residues in regions of secondary structure. A similar approach was taken using an ensemble of structures from the MD simulations to calculate electronic circular dichroism (CD) and IR spectra from first principles with an encouraging correlation with the experimental CD and IR data. Proteins 2008. (c) 2007 Wiley-Liss, Inc.

About this Structure

2K0B is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Conformation and dynamics of the three-helix bundle UBA domain of p62 from experiment and simulation., Evans CL, Long JE, Gallagher TR, Hirst JD, Searle MS, Proteins. 2007 Oct 11;71(1):227-240. PMID:17932931

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