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Publication Abstract from PubMed

Mutants of human Cellular Retinol Binding Protein II (hCRBPII) were engineered to bind a julolidine retinal analog for the purpose of developing a ratiometric pH sensor. The design relied on the electrostatic influence of a titratable amino acid side chain, affecting the absorption, and thus emission of the protein/fluorophore complex. The ratio of emissions obtained at two excitation wavelengths that correspond to the absorption of the two forms of the protein/fluorophore complex, leads to a concentration independent measure of pH.

A Genetically Encoded Ratiometric pH-Probe: Wavelength Regulation-Inspired Design of pH Indicators.,Berbasova T, Tahmasebi Nick S, Nosrati M, Nossoni Z, Santos E, Vasileiou C, Geiger J, Borhan B Chembiochem. 2018 Apr 12. doi: 10.1002/cbic.201800050. PMID:29645331^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.